恒温扩增检测结核分枝杆菌特异性IS6110片段在肺结核检测中的应用  

The Clinical Application of DNA Loop Mediated Isothermal Amplification Technology for Detecting IS6110 in the Diagnosis of Pulmonary Tuberculosis

作　　者：李玉雪[1] 王薇[1] 柳晓金[2] 范蓉[1] 安翠平[1] 

机构地区：[1]石家庄市第一医院检验科,河北石家庄050011 [2]石家庄市第五医院检验科,河北石家庄050000

出　　处：《标记免疫分析与临床》2017年第11期1310-1312,共3页Labeled Immunoassays and Clinical Medicine

基　　金：石家庄市科技支撑计划项目(131462533)

摘　　要：目的比较恒温扩增技术检测、抗酸杆菌涂片、结核分枝杆菌培养在肺结核患者检测中的应用。方法收集2016年1月1日至12月31日我院呼吸科肺结核患者清晨痰液,将标本分为3份,分别进行痰涂片抗酸染色,结核菌培养及恒温扩增检测结核分枝杆菌特异性IS6110片段,统计分析3种方法的检测结果。结果 56例痰标本,恒温扩增检测结核分枝杆菌特异性IS6110片段阳性33例,阳性率为58.93%,抗酸染色阳性15例,阳性率为26.79%,恒温扩增检测结核分枝杆菌特异性IS6110片段阳性率高于抗酸染色方法;结核分枝杆菌培养阳性为35例,阳性率62.50%;恒温扩增检测结核分枝杆菌特异性IS6110片段和结核分枝杆菌培养两种方法检测Kappa系数为0.776,检测一致性较好。结论恒温扩增检测结核分枝杆菌特异性IS6110片段是一种简便、高灵敏度的检出结核分枝杆菌的方法。Objective To compare the clinical application of DNA loop mediated isothermal amplification （LAMP） technology, sputum smear microscopy and culture in the diagnosis of pulmonary tuberculosis. Methods Sputum samples of patients clinically diagnosed with pulmonary tuberculosis from 1 January 2016 to 31 December 2016 were performed withLAMP technology for detecting IS6110 ,direct smear microscopy and culture. Results In 56 specimens, the positive rates of LAMP technology, direct smear microscopy and culture were 58.39% （ 33/56 ）, 26.79% （15/56） and 62, 50% （ 35/56 ）, respectively. The positive rare of LAMP technology was higher than that of direct smear microscopy. And the differences were statistically significant （ P 〈 0.05 ）. The consistency between LAMP technology and culture was good （ Kappa = 0. 776）. Conclusion In the diagnosis of pulmonary tuberculosis, the LAMP technology is convenient, and has higher sensitivity.

关 键 词：肺结核 结核分枝杆菌 核酸扩增技术 

分 类 号：R446.5[医药卫生—诊断学] R521[医药卫生—临床医学]