烟草根黑腐病菌ISSR标记的遗传多样性分析  

Genetic diversity analysis of Thielaviopsis basicola by ISSR method

作　　者：窦彦霞[1] 徐林 马冠华[1] 李六英 张世渠 

机构地区：[1]西南大学植物保护学院重庆市北碚天生路2号,400716 [2]重庆市烟草公司彭水分公司重庆市彭水县汉葭镇北门街,409600

出　　处：《烟草科技》2017年第11期9-15,共7页Tobacco Science & Technology

基　　金：中央高校基本科研业务费专项项目“根串珠霉ds RNA病毒多样性研究”(XDJK2015C167);重庆市烟草专卖局资助项目“烟草黑胫病菌和赤星病菌致病性分化和病害流行规律研究”(NY20140401070002)

摘　　要：为从分子水平揭示烟草根黑腐病菌的群体遗传学特性,应用简单重复性序列间区(ISSR)分子技术对我国重庆、甘肃、贵州、河南及云南5个不同烟草根黑腐病菌地理种群54株菌株进行了遗传多样性和分化研究。结果表明:用筛选的5个多态性引物扩增ISSR条带平均为9.6条(8~12条),其中多态性条带平均3.6条(2~5条)。总样本Nei's指数h为0.350 7,香农指数I为0.521 5;基因分化系数GST为0.338 9,基因流Nm为0.975 6。5个种群的遗传距离在0.113 4~0.275 2之间,河南种群和其他4个地理种群的遗传相似度均较高,遗传距离较近;贵州种群除与河南种群遗传相似度较高外(0.804 8),与其余种群的相似度均较低。NTsys 2.10e聚类分析结果显示,不同菌株遗传相似系数为0.82~1.00,当相似系数为0.84时,菌株聚为5支。说明我国烟草根黑腐病菌基因多样性较高,不同种群存在遗传分化现象,种群间基因流动大。To study the population genetics of Thielaviopsis basicola at molecular level, 54 isolates of T. basicola were collected from Chongqing, Gansu, Guizhou, Henan and Yunnan, and the genetic diversity and population differentiation among geographic populations were analyzed by ISSR method. The results showed that the average of bands amplified by 5 ISSR primers pairs was 9.6 （8-12） and the average of polymorphism bands was 3.6 （2-5）. The Nei＇s index h, Shannon index I, G ST value and the gene flow Nm of total populations were 0.350 7, 0.521 5, 0.338 9 and 0.975 6, respectively. The genetic distances of the 5 populations ranged from 0.113 4 to 0.275 2. The genetic identities between Henan and the other populations were higher with close genetic distances. The genetic identity of Guizhou population with Henan population was higher （0.804 8）, with the other populations was lower. NTsys 2.10e cluster analysis indicated that the genetic similarity coefficients of different isolates were from 0.82 to 1.00 and the isolates could be clustered into 5 groups at the genetic similarity coefficient of 0.84. In conclusion, T. basicola maintains rich genetic diversity in China, there exists genetic differentiation among different populations, and the gene flow in populations was large.

关 键 词：烟草根黑腐病菌 简单重复性序列间区(ISSR) 遗传多样性 遗传分化 

分 类 号：S432.41[农业科学—植物病理学]