血液增菌肉汤连续传代对多药耐药菌恢复抗菌药物敏感性研究  

Recovery of sensitivity of multidrug-resistant bacteria to antibacterial drugs by continuous regeneration of blood-enhanced broth

作　　者：田恒忠 孟祥清[2] 王健[3] 孙丹[2] 邢浩莉 赵淑海[5] 倪宁[1] 宋慧 康栗[2] 李倩[7] 

机构地区：[1]淮南市妇幼保健院儿科,安徽淮南232007 [2]淮南朝阳医院检验科,安徽淮南232007 [3]安徽理工大学病原生物学与免疫学教研室,安徽淮南232007 [4]淮南市妇幼保健院院感办,安徽淮南232007 [5]淮南市妇幼保健院院部,安徽淮南232007 [6]淮南市妇幼保健院医务科,安徽淮南232007 [7]淮南市妇幼保健院检验科,安徽淮南232007

出　　处：《中华医院感染学杂志》2017年第21期4826-4829,共4页Chinese Journal of Nosocomiology

基　　金：淮南市科技局基金资助项目(2016A26(2))

摘　　要：目的探讨连续传代培养对产ESBLs多药耐药菌恢复药物敏感性的影响。方法选择临床产ESBLs的大肠杆菌和肺炎克雷伯杆菌各21株,以血液增菌肉汤连续30代接种传代。对连续传代且无杂菌污染的菌株进行体外药敏试验。结果共获得纯化的产ESBLs的大肠杆菌和肺炎克雷伯杆菌分别为15株和18株,传30代结果显示共有21株51种次的抗菌药物由耐药变成敏感,其敏感性恢复了,4株6种次由敏感变成耐药。结论血液增菌肉汤连续30代接种传代可诱导部分产ESBLs肺炎克雷伯菌、大肠埃希菌恢复药物敏感性。OBJECTIVE To investigate the effect of continuous subculture on the recovery of drug sensitivity of ESBLs-producing multidrug-resistant bacteria.METHODS A total of 21 strains of ESBLs-producing Escherichia coli and 21 strains of ESBLs-producing Klebsiella pneumoniae were selected,and the blood enhanced broth was inoculated for 30 generations.The in vitro drug susceptibility test was carried out for strains which were continuously passaged and had no contamination.RESULTS A total of 15 strains of purified ESBLs-producing E.coli and 18 strains of purified ESBLs-producing K.pneumoniae were obtained.The results showed that,after 30 generations of subculture,21 strains of 51 species were sensitive to antibacterial drugs and their sensitivity was restored,and4 strains of 6 species turned from sensitivity to resistance.CONCLUSIONBlood-enhanced broth can induce partial ESBLs-producing K.pneumoniae and E.coli to restore drug sensitivity after 30 generations of subculture.

关 键 词：多药耐药菌 血液增菌肉汤 敏感性恢复 

分 类 号：R978.1[医药卫生—药品]