聚维酮碘消毒剂对金黄色葡萄球菌生物膜的体外影响研究  

In vitro antibacterial effect of povidone-iodine disinfectant on biofilm of Staphylococcusaureus

作　　者：罗延智 曾华[2] 张志宏[1] 孙效棠[1] 王万明[1] 

机构地区：[1]南京军区福州总医院骨二科,福建福州350025 [2]南京军区福州总医院感控办,福建福州350025

出　　处：《中华医院感染学杂志》2017年第21期5010-5013,共4页Chinese Journal of Nosocomiology

基　　金：福建省自然科学基金资助项目(2014J01369);全军医药卫生科研基金资助项目(15DX022)

摘　　要：目的观察聚维酮碘消毒剂对浮游状态金黄色葡萄球菌以及钛合金表面金黄色葡萄球菌生物膜的体外抗菌效果,为临床研究提供参考。方法采用细菌悬液定量杀灭实验,观察聚维酮碘对浮游状态金黄色葡萄球菌的抗菌活性;在钛合金盘表面构建24小时细菌生物膜模型,通过激光共聚焦显微镜及扫描电镜观察细菌生物膜结构;采用活菌计数法,观察聚维酮碘对钛合金表面的细菌生物膜的抗菌活性。结果实验室条件下,有效碘浓度为0.1%、0.2%、0.3%、0.4%以及0.5%的聚维酮碘溶液作用30秒均可全部杀灭浮游状态的金黄色葡萄球菌;在激光共聚焦显微镜及扫描电镜下均观察到成熟的细菌生物膜;经过聚维酮碘浸泡作用5min后,同对照相比,随着聚维酮碘浓度的不断升高,钛合金盘上的CFU活菌计数不断下降,0.5%聚维酮碘对细菌生物膜浸泡作用10min后,CFU计数为0。结论实验室条件下,聚维酮碘不仅对浮游状态金黄色葡萄球菌具有强大的抗菌活性,对钛合金表面的24小时金葡菌生物膜同样具有抗菌活性,且抗菌活性随着聚维酮碘浓度的增加及作用时间的延长而增强,提示应用0.5%的聚维酮碘原液浸泡>10分钟才能彻底杀灭生物膜内的细菌。OBJECTIVE To observe the in vitro antibacterial effect of povidone-iodine disinfectant on biofilms of planktonic state of Staphylococcus aureus and the S.aureus on surfaces of titanium alloy plate so as to provide guidance for clinical study.METHODS By means of suspension quantitative germicidal test,the antibacterial activity of povidone-iodine against the planktonic state of S.aureus was observed,the 24-hour bacterial biofilm model was established on the surfaces of titanium alloy plate,the structure of bacterial biofilm was observed by using laser scanning confocal microscope and scanning electron microscope,and the antibacterial activity of povidone-iodine against the bacterial biofilms on the surfaces of titanium alloy plate was observed by means of viable counting method.RESULTS Under laboratory conditions,all planktonic S.aureus strains could be killed in 30 seconds with povidone-iodine with the available iodine concentrations of 0.1%,0.2%,0.3%,0.4% and 0.5%.The mature biofilms could be displayed under both CLSM and SEM.After 5 min of immersion in povidone-iodine,the CFU viable counts on titanium alloy disk were constantly decreased with the increase of concentration of povidone-iodine,and the CFU count was 0 after the biofilms were immersed in 0.5% povidone-iodine for 10 min.CONCLUSION The povidone-iodine has antibacterial activity against not only the planktonic state of S.aureus but also the 24-hour biofilms of S.aureus on the surfaces of titanium alloy plate,the antibacterial activity is enhanced with the increased of concentration of povidone-iodine and extension of effecting time,indicating that the bacteria within the biofilm can be completely killed after more than 10 min of immersion in 0.5% povidone-iodine.

关 键 词：聚维酮碘 浮游菌 生物膜 金黄色葡萄球菌 

分 类 号：R378.11[医药卫生—病原生物学]