FPS-ZM1拮抗晚期糖基化终末产物受体逆转高糖所致的牙周膜成纤维细胞炎症反应  

FPS-ZM1 Rescues the Inflammatory Response of hPDLFs Caused by High Glucose via Inhibition of RAGE

作　　者：詹聃婷 丁农乐 张云水 张慧 郭玲 

机构地区：[1]西南医科大学附属口腔医院修复科,四川泸州646000

出　　处：《口腔医学研究》2017年第11期1156-1160,共5页Journal of Oral Science Research

基　　金：四川省科技厅面上项目(编号:2017JY0111);泸州市-校联合(编号:2016LZXNYD-J20)

摘　　要：目的:以体外培养的人牙周膜成纤维细胞(hPDLFs)为研究对象,探讨新型小分子RAGE特异性抑制剂对高糖环境培养下牙周膜成纤维细胞炎性因子的影响。方法:原代培养hPDLFs,Western-Blot检测高糖对RAGE表达的影响,RT-PCR检测FPS-ZM1对高糖环境下IL-6和TNF-α基因表达的影响,ELISA检测IL-6和TNF-α的蛋白分泌变化。结果:高糖可导致hPDLFs表面RAGE表达增加,加重细胞炎症反应。而FPS-ZM1可以下调IL-6和TNF-α的基因表达,同时减少炎症介质IL-6和TNF-α的分泌。结论:RAGE特异性抑制剂FPS-ZM1可以在一定程度上逆转高糖刺激导致hPDLFs产生的炎症反应。Objective：To observe the effect of FPS-ZM1 on IL-6 and TNF-αexpression of human periodontal ligament fibroblasts（hPDLFs）cultured in high glucose.Methods：hPDLFs were primarily cultured and exposed to either low glucose（5.5 mmol/L）or high glucose（25 mmol/L）.Under high glucose,the expression of RAGE was measured by Western-blot.The gene and protein expressions of IL-6 and TNF-αwere measured by RT-PCR and ELISA under high glucose with various concentration of FPS-ZM1.Results：High glucose could enhance RAGE expression and the inflammatory response of hPDLFs,which was inverted by RAGE inhibitor FPS-ZM1.Conclusion：FPS-ZM1 can rescue the inflammatory response of hPLDFs caused by high glucose.

关 键 词：牙周膜成纤维细胞 RAGE抑制剂 高糖 炎症反应 

分 类 号：R587.1[医药卫生—内分泌] R781.4[医药卫生—内科学]