虾夷扇贝种贝促熟期饵料贡献率研究  

Diet Contribution Proportion during Broodstock Maturation in Japanese Scallop Patinopecten yessoensis

作　　者：田甲申[1] 李多慧 王摆[1] 鹿志创[1] 乔壮 韩家波[1] 

机构地区：[1]辽宁省海洋水产科学研究院,辽宁省海洋生物资源和生态学重点实验室,辽宁大连116023 [2]大连市水产研究所,辽宁大连116019 [3]大连花园口渔港监督,辽宁大连116023

出　　处：《水产科学》2017年第6期722-727,共6页Fisheries Science

基　　金：“十二五”农村领域国家科技计划项目(2013BAD23B01);辽宁省海洋与渔业厅科研项目(201609)

摘　　要：应用稳定同位素技术检测了促熟期间虾夷扇贝种贝不同组织及7种投喂饵料的碳、氮稳定同位素比值。结果显示,饵料的δ15 N变化为2.584‰~7.230‰,跨度为4.646‰;δ13 C变化为-24.701‰^-16.365‰,跨度为8.336‰。雌性种贝各组织的δ13 C由高到低依次为:闭壳肌、外套膜、性腺、鳃;雄性的为:闭壳肌、外套膜、鳃、性腺。采用IsoSource线性混合模型对7种饵料的贡献率进行了分析,结果发现,投喂饵料对促熟期雌、雄种贝的饵料贡献率基本一致;饵料对促熟期雌、雄种贝性腺发育的贡献率存在显著差异,对雄性性腺发育的平均贡献率由高到低依次为:湛江叉鞭金藻(20.5%)、鸡蛋黄(20.2%)、酵母(14.3%)、小新月藻(13.8%)、青岛大扁藻(12.1%)、盐藻粉(11.0%)、螺旋藻粉(8.1%);对雌性性腺发育的平均贡献率由高到低依次为:鸡蛋黄(45.0%)、湛江叉鞭金藻(22.3%)、酵母(8.2%)、小新月藻(7.8%)、青岛大扁藻(6.7%)、盐藻粉(5.9%)、螺旋藻粉(4.1%)。试验结果表明,雌、雄种贝性腺发育期的营养需求存在差异。上述研究结果有助于合理制定虾夷扇贝种贝促熟期的饵料投喂策略。The carbon （δ13C） and nitrogen （31SN） stable isotope values were investigated in different tis- sues in Japanese scallop Patinopecten yessoensis and 7 diets during broodstock maturation. The results demonstrated that 31SN value in diets was ranged from 2. 584%0 to 7. 230%0 while the counterpart in 813 C ranged from --24. 701%0 to --16. 365%o. The maximal value of 313C was observed in adductor muscle, fol- lowed by mantle, gonad and gill in female broodstock, while in male broodsotck the difference was only between gill and goand. Then, the 7 dietslcontribution to Japanese scallop were analyzed by the application of IsoSource linear multiple models. The results showed that there was similar in the contribution propor- tion between male and female broodstock fed diets the difference in diets contribution proportion between male and female gonads was significant difference during the broodstock maturation. The maximal contri- bution proportion for the male gonad was alga Dicrateria zhanjiangensis （20. 5 %）, followed by egg yolk （20. 2%）, yeast powder （14. 3%）, alga Closterium venus （13. 8%）, alga Platymonas helgolandica （12. 1%）, alga Dunaliella salina powder （11.0%） and alga Spirulina powder （8. 1%）. The maximal contribution proportion for the female gonad was egg yolk （45. 0%）, followed by D. zhanjiangensis （22.3%）, yeast powder （8. 2%）, C. venus （7. 8%）, P. helgolandica （6.7%）, Dunaliella salina powder （5.9%） and Spirulina powder （4. 1%）. The results showed that there was difference in nutritional re- quirement between male and female scallop during the gonad maturation. Moreover, these findings could be beneficial for making strategy for feeding Japanese scallop during broodstock maturation.

关 键 词：虾夷扇贝 碳氮稳定同位素 贡献率 不同组织 

分 类 号：S968.31[农业科学—水产养殖]