普通小麦籽粒过氧化物酶活性全基因组关联分析  

Genome-Wide Association Study of Grain Peroxidase Activity in Common Wheat

作　　者：时佳[1] 翟胜男 刘金栋[2] 魏景欣 白璐[3] 高文伟[1] 闻伟锷 何中虎[2] 夏先春[2] 耿洪伟[1] 

机构地区：[1]新疆农业大学农学院/新疆农业大学生物技术重点实验室,乌鲁木齐830052 [2]中国农业科学院作物科学研究所/国家小麦改良中心,北京100081 [3]新疆农业大学科学技术学院,乌鲁木齐830091

出　　处：《中国农业科学》2017年第21期4212-4227,共16页Scientia Agricultura Sinica

基　　金：国家自然科学基金(31771786);2016年南京农业大学-新疆农业大学联合基金(KYYJ201602)

摘　　要：【目的】小麦籽粒过氧化物酶(peroxidase,POD)活性对面制品加工品质有重要影响,发掘控制籽粒POD活性重要位点,并筛选其候选基因,为小麦品质的改良奠定基础。【方法】以151份黄淮冬麦区和82份北部冬麦区品种(系)为材料,分别利用来自于小麦90 K SNP芯片的18 189和18 417个高质量SNP标记,对POD活性进行全基因组关联分析(genome-wide association study,GWAS)。【结果】供试材料中POD活性表现出广泛的表型变异和多样性,黄淮麦区材料的POD活性变异系数为15.4%—21.8%,遗传力为0.79,北部麦区材料的POD活性变异系数为15.0%—19.9%,遗传力为0.82。相关性分析表明,不同环境之间材料的POD活性表现出显著的相关性,黄淮麦区相关系数为0.46—0.89(P<0.0001),北部麦区相关系数为0.50—0.87(P<0.0001)。多态性信息含量PIC值为0.09—0.38,最小等位基因频率MAF值为0.05—0.5。群体结构分析表明,黄淮麦区与北部麦区2个自然群体结构简单,均可分为3个亚群。GWAS分析结果表明,在黄淮冬麦区材料中共检测到20个与POD活性显著关联的位点(P<0.001),分布在1A、2A、2B、2D、3A、3B、3D、4A、4B、5A、5B、6A、6D和7A染色体上,单个位点可解释7.8%—13.3%的表型变异。在北部冬麦区材料中共检测到20个与POD活性显著关联(P<0.001)的位点,分布在1A、1B、1D、2A、2B、2D、3A、3B、4B、6A、6B、7A、7B和7D染色体上,单个位点可解释14.4%—23.2%的表型变异。加性回归分析表明,随着优异等位基因数量的增多,小麦籽粒POD活性越高。在发现的所有POD活性相关位点中,2个位点在黄淮麦区和北部麦区材料中均能检测到且稳定遗传,可将其转换为STARP(semi-thermal asymmetric reverse PCR)或CAPS标记,以应用于分子标记辅助育种。获得3个与POD活性有关的候选基因,分别编码磷酸甘露糖变位酶(PMM-D1)、辣根过氧化物酶(PER40)和烷基氢过氧化物还原酶(F775_31640)。【结论】【Objective】Peroxidase（POD） activity has browning and bleaching effects on the color of flour and flour-based products during processing and storage.Identification of associated loci and candidate genes for grain POD activity is important for molecular marker-assisted selection（MAS） in wheat quality breeding.【Method】In the present study,the POD activities were surveyed with 151 and 82 Chinese bread wheat cultivars from Yellow Huai Winter Wheat Region（YHRVWWR） and Northern China Plain Winter Wheat Region（NWWR）,respectively,and each set of cultivars was planted in four environments.A genome-wide association study（GWAS） was performed using the mixed linear model（MLM） based on 18 189 and 18 417 high-quality SNP markers from 90 K SNP array for two sets of cultivars,respectively.【Result】The POD activity of the tested materials showed extensive phenotypic variation and diversity.The variation coefficient of YHRVWWR was 15.4%-21.8%,the heritability was 0.79,and the variation coefficient of NWWR was 15.0%-19.9%,the heritability was 0.82.The POD activity of the materials in different environments showed a significant correlation,and the correlation coefficients were 0.46-0.89（P〈0.0001） and 0.50-0.87（P〈0.0001） in YHRVWWR and NWWR,respectively.The polymorphic information content of value was between 0.09-0.38,and the minimum allele frequency was between 0.05-0.5.The population structure analysis showed that the two natural populations in YHRVWWR and NWWR were simple and could be divided into three subgroups.In the YHRVWWR cultivars,20 loci were found to be associated with POD activity（P〈0.001）,which were located on chromosomes 1 A,2 A,2 B,2 D,3 A,3 B,3 D,4 A,4 B,5 A,5 B,6 A,6 D and 7 A,and each explained 7.8%-13.3% of phenotypic variation.In the NWWR cultivars,20 loci showed significant association with POD activity（P〈0.001）,which were located on chromosomes 1 A,1 B,1 D,2 A,2 B,2 D,3 A,3 B,4 B,6 A,6 B,7 A,7 B and 7 D,explaining 14.4%-23.2% of phenotypic variation.Two

关 键 词：普通小麦 POD活性 90 K SNP芯片 群体结构 候选基因 

分 类 号：S512.1[农业科学—作物学]