主客体结构微球的流式免疫荧光检测的方法  

Research on Immunofluorescent Detection Method by Flow Cytometry Based on Bead-on-Bead Structured Particles

作　　者：吴志民 徐宏[1] 古宏晨[1] 

机构地区：[1]上海交通大学生物医学工程学院,上海200030

出　　处：《上海交通大学学报》2017年第11期1361-1366,共6页Journal of Shanghai Jiaotong University

基　　金：国家高技术研究发展计划(863)项目(2012AA020103);上海市经信委产学研课题项目(CXY-2013-56)资助

摘　　要：采用纳米氧化硅客体子球和聚苯乙烯磁性主体母球设计并制备了一种新型的主客体组装结构复合微球,将抗人绒毛膜促性腺激素(hCG)抗体化学固载于组装微球表面,在流式免疫荧光检测平台中研究其免疫检测性能,并与传统的酶联免疫(ELISA)检测方法进行了比较.结果表明,主客体组装结构微球在流式免疫荧光检测中具有优异的检测性能,在微球用量极低的情况下仍然具有非常好的检测线性和检测重复性,并且空白限较传统的ELISA方法降低了6/7.A new type host-guest structured bead-on-bead （BOB） particle was designed and synthesized via covalent assembling guest nano silica particles onto host magnetic polymer particles. Using BOB particles as biocarrier, anti-human chorionic gonadotrophin （hCG） antibody was covalently immobilized on the sur- face of BOB particles. Then, the immunofluorescent detection capability in recognition hCG antigen on flow cytometry was studied and compared with traditional enzyme-linked immuno sorbent assay （ELISA）. The result showed that BOB particles presented remarkable immunofluorescent detection capability on flow cytometry. BOB particles also showed excellent linear detection than traditional ELISA detection in ex- tremely low particles amount. Further more, the limit of blank （LOB） of immunofluorescent detection on flow cytometry was nearly 1/7 to traditional ELISA detection.

关 键 词：主客体组装结构微球 生物载体 流式免疫荧光检测 酶联免疫检测 

分 类 号：TB34[一般工业技术—材料科学与工程]