阿托伐他汀对泡沫细胞载脂蛋白M表达的影响  

Effect of Atorvastatin on the Expression of Apolipoprotein M

作　　者：刘健[1] 虞敏[1] 王利民[1] 施盛[1] 袁忠祥[1] 

机构地区：[1]上海交通大学附属第一人民医院心脏大血管外科,上海市210080

出　　处：《中国分子心脏病学杂志》2017年第5期2247-2250,共4页Molecular Cardiology of China

基　　金：上海市卫计委面上项目(201440293)

摘　　要：目的观察阿托伐他汀对THP-1巨噬细胞源性泡沫细胞载脂蛋白M(ApoM)表达的影响。方法培养THP-1单核细胞,序贯加入佛波酯(PMA)和氧化低密度脂蛋白(ox-LDL)进行诱导,使其分化为泡沫细胞,RT-qPCR和油红O染色鉴定。泡沫细胞模型建立后,加入阿托伐他汀处理,RT-qPCR和Western blot检测ApoM的m RNA和蛋白的表达变化,使用人pre-β-HDL酶联免疫试剂盒检测细胞培养基中pre-β-HDL水平。结果阿托伐他汀处理后的泡沫细胞较对照组和DMSO组ApoM m RNA和蛋白表达均有升高(P<0.05)。与对照组和DMSO组细胞上清中pre-β-HDL表达量相比较,阿托伐他汀组上清中pre-β-HDL表达量有明显升高(P<0.05)。结论阿托伐他汀能上调泡沫细胞ApoM的表达,继而刺激pre-β-HDL的合成和分泌,促进胆固醇从泡沫细胞内流出。ApoM可能是他汀类药物的重要降脂机制之一。Objective To investigate the effect of atorvastatin on the expression of apolipoprotein M（ApoM）in foam cells.Methods The THP-1 cells were cultured and incubated with phorbool-1-myristate-13-acetate（PMA）and oxidized low density lipoprotein（ox-LDL）to establish foam cell model.RT-qPCR and oil red O staining were used to identify foam cells.RT-qPCR and Western blot were performed to analyze the m RNA and protein expression levels of ApoM in atorvastatin treated foam cells.The concentration of pre-β-HDL in culture media was measured by human pre-β-HDL enzyme-linked immunosorbent assaykit.Results Compared with the control group and the DMSO group,Atorvastatincan statistically increase the expression of ApoM（P〈0.05）.The level of pre-β-HDLin culture media of atorvastatin treated foam cells is significantly higher than the other two groups.Conclusions Hypolipidemic effect of atorvastatin may be attributed to up-regulating of ApoM expression.

关 键 词：泡沫细胞 阿托伐他汀 胆固醇逆向转运 载脂蛋白M 

分 类 号：R96[医药卫生—药理学]