正交设计与Box-Behnken设计比较研究优化岩高兰多糖提取工艺  

Comparative Orthogonal Design and Box-Behnken Design Optimization of Extraction Technology of Empetrum Nigrum L.for Polysaccharide

作　　者：刘寒[1] 金司阳 郭慧[1] 王振月[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药导报》2017年第21期71-74,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:比较研究两种实验设计方法对岩高兰多糖提取率的影响,以获得最大多糖溶出率。方法:以岩高兰多糖提取率为考察指标,采用正交设计法和Box-Behnken设计法优化提取工艺并对结果进行统计分析。结果:Box-Behnken设计法得到的多糖提取率比正交试验设计高。结论:岩高兰多糖提取率的最优工艺以BoxBehnken设计法为准,为粉碎粒度100目,时间1.75 h,电机电压155 V,料液比1∶15,提取2次。Objective： To optimize extraction rate technology of polysaccharide. Methods： The polysaccharide extracting rate was the investigation index, through the method of orthogonal design and Box-Behnken design optimization of Empetrum nigrum polysaccharide extraction process research, and then the experimetal results of the two methods were analyzed. Results： Box-Behnken design method showed higher polysaccharide digestion rate than the orthogonal design. Conclusion： The optimum extraction of polysaccharide from Empetrum nigrum L. was smashed herbs to pass through 100-mesh scream, add 15 timesparts of water, voltage 1.75 h under 155 V two times.

关 键 词：岩高兰 正交设计 BOX-BEHNKEN设计 多糖 提取工艺 

分 类 号：R284[医药卫生—中药学]