抗-HI抗体的筛查和鉴定在临床输血中的应用价值  

Value of anti-HI antibody screening and identification in clinical blood transfusion

作　　者：马春娅[1] 张晓娟[1] 王金慧[1] 刘晓敏[1] 刘洋[1] 于洋[1] 汪德清[1] 

机构地区：[1]解放军总医院输血科,北京100853

出　　处：《国际检验医学杂志》2017年第22期3094-3096,共3页International Journal of Laboratory Medicine

基　　金："十三五"全军后勤科研重点项目子课题(BWS16J006)

摘　　要：目的探讨抗-HI抗体的筛查和鉴定在临床输血中的应用价值。方法选取2016年2-11月该院4例抗-HI抗体阳性患者作为研究对象,采用抗人球蛋白法和盐水试管法进行不规则抗体的筛查和鉴定,并对临床输血效果进行评价。结果 4例患者均为ABO血型,其中A型RhD阳性2例,AB型RhD阳性2例。抗人球蛋白法的筛查结果均为弱阳性,盐水试管法的筛查结果均为阳性。在盐水介质条件下,4例患者血清与16系谱细胞反应结果均为阳性,与自身红细胞反应结果均为阴性;在抗人球蛋白介质条件下,4例患者血清与16系谱细胞反应结果除1例弱阳性外均为阴性,与自身红细胞反应结果均为阴性。2例治疗用血患者输血后,血红蛋白上升达到预期效果,无输血不良反应发生。结论为抗-HI抗体阳性患者输血时,应选择ABO同型且盐水试管法及抗人球蛋白法配血结果均相合的血液。Objective To investigate the value of anti-HI antibody screening and identification in clinical blood transfusion.Methods 4 cases of anti-HI antibody positive in our hospital from February 2016 to November were selected as the research subjects.The irregular antibodies screening and identification were performed by adopting the anti-human globulin and saline test tube method,then the blood transfusion effect was evaluated.Results Four cases were ABO blood group,in which 2 cases were group A RhD positive and 2 cases were group AB RhD positive.The screening results of anti-human globulin method were weak positive,while which of saline test tube method were positive.Under the saline medium condition,the reaction results of serum with 16 spectrum cellular reaction in 4 cases were positive,while which with autoerythrocytes were negative;under the anti-human globulin medium condition,the reaction results of serum with 16 spectrum cellular reaction in 4 cases were negative except 1 case of weak positive,while which with autoerythrocytes were negative.After blood transfusion in 2 cases of treatment blood use,Hb increase reached the expected effect without blood transfusion adverse reactions occurrence.Conclusion In blood transfusion for the patients with anti-HI antibody positive,the blood of same type ABO and compatible results in both saline test tube method and anti-human globulin tests should be selected.

关 键 词：抗-HI IgM类抗体 输血效果 

分 类 号：R446.6[医药卫生—诊断学] R457.1[医药卫生—临床医学]