应用拮抗试验分析灵芝菌株遗传多样性  

Analysis of the genetic diversity of Ganoderma lucidum strains based on antagonism

作　　者：李倩芸 李荔清 张维瑞 刘盛荣 

机构地区：[1]宁德师范学院生命科学学院,福建宁德352100

出　　处：《食药用菌》2017年第6期363-365,共3页Edible and Medicinal Mushrooms

基　　金：福建省教育厅项目(编号JA15563);宁德师范学院服务海西项目(编号2013F22)

摘　　要：收集25株赤芝及5个灵芝属的非赤芝菌株作为供试菌株,采用拮抗试验分析灵芝(赤芝)菌种的遗传多样性。结果:25株赤芝全随机300个配对中291个配对产生拮抗反应,占97.00%。其中151对形成拮抗线,占50.33%;98对形成隆起拮抗反应,占32.67%;42对形成沟状反应,占14.00%;有9个配对无拮抗反应,占3.00%。另还发现4株赤芝与其他非赤芝菌株未形成拮抗反应。表明我国灵芝(赤芝)菌株具有丰富的遗传多样性。A total of 30 Ganoderma strains （25 strains belonging to G. lucidum） were used for antagonism test to analyze the genetic diversity of G lucidum strains in China. The results demonstrated that, out of 300 pairings among 25 G. lucidum strains,＇291 formed antagonism reaction with the ratio of 97.00% （151 pairings produced line at 50.33% ratio, 98 pairings were bulge at 32.67%, 42 pairings were barrage at the ratio of 14.00%）. The remaining 9 pairings at 3.00% did not show antagonism. In addition, no antagonism was observed for 4 pairing between G. lucidum strains and other Ganoderma strains. These findings proved that there is an abundant genetic diversity within G. lucidum strains in China.

关 键 词：灵芝 拮抗试验 遗传多样性 种质资源 

分 类 号：S567.31[农业科学—中草药栽培]