左归饮对D-半乳糖致衰大鼠肝肾组织形态及其细胞凋亡相关蛋白表达的影响  

Effects of Zuogui Drink on morphology of liver and kidney tissues and expression of apoptosis related proteins in D-galactose induced aging rats

作　　者：周寅 杨绍杰[1] 陈光伟 纪锐 孙佳宝 杨书彬[1] 

机构地区：[1]黑龙江中医药大学药学院,黑龙江哈尔滨150040

出　　处：《上海中医药杂志》2017年第11期72-78,共7页Shanghai Journal of Traditional Chinese Medicine

基　　金：全国中药特色技术传承人才培训项目资助;黑龙江中医药大学研究生创新科研项目(YJSCX2016045)

摘　　要：目的研究左归饮对D-半乳糖致亚急性衰老模型大鼠肝、肾组织形态及其细胞凋亡相关蛋白表达的影响,初步探讨其延缓衰老作用机制。方法取健康SD大鼠72只,雌雄各半,随机分为6组,即对照组、模型组、给药组(维生素E组,左归饮高、低剂量组和六味地黄丸组)。除对照组颈背部皮下注射0.9%Na Cl溶液外,其余各组颈背部皮下分别连续注射D-半乳糖建立亚急性衰老模型。建模同时,各给药组大鼠分别灌胃给予维生素E、高剂量左归饮、低剂量左归饮及六味地黄丸药液进行干预,对照组与模型组分别给予等体积的蒸馏水。连续给药8周后,取大鼠肝、肾组织固定,利用HE染色法制作病理切片,进行显微观察;同时,采用免疫组化方法检测肝、肾组织中细胞凋亡相关蛋白(Bax、Bcl-2、Caspase-3)阳性表达水平,统计比较IOD值。结果 (1)与对照组比较,模型组大鼠肝、肾组织病理性损伤明显;与模型组比较,左归饮能够减轻D-半乳糖致大鼠衰老过程中出现的肝、肾组织病理性损伤。(2)与对照组比较,模型组大鼠肝组织中Bax和Caspase-3的IOD值均显著升高(P<0.01),Bcl-2的IOD值显著降低(P<0.01);与模型组比较,左归饮高剂量组大鼠肝组织中Bax、Caspase-3的IOD值均显著降低(P<0.01),Bcl-2的IOD值显著升高(P<0.01)。左归饮低剂量组大鼠肝组织中Bax和Caspase-3的IOD值明显降低(P<0.05),Bcl-2的IOD值略有升高,但差异无统计学意义(P>0.05)。(3)与对照组比较,模型组的肾组织中Bax和Caspase-3的IOD值均显著升高(P<0.01),Bcl-2的IOD值显著降低(P<0.01);与模型组比较,左归饮高剂量组大鼠肾组织中Bax、Caspase-3的IOD值均明显降低(P<0.01),Bcl-2的IOD值明显升高(P<0.01)。左归饮低剂量组大鼠肾组织中Bax的IOD值明显降低(P<0.05),但Bcl-2及Caspase-3的IOD值差异无统计学意义(P>0.05)。结论左归饮对肝、肾组织具有一定的保护作用,其作用机制可能是通过与影�Objective To study the effects of Zuogui Drink on the morphology of liver and kidney tissues and expression of apoptosis related proteins in D-galactose induced subacute aging rats,and discuss its mechanism of anti-aging. Methods 72 healthy SD rats（ half male and half female） were randomly divided into the control group,model group,vitamin E group,Liuwei Dihuang Pill group and Zuogui Drink groups with high-and low-dose. Except the control group that treated with 0. 9% Na Cl solution by subcutaneous injection at the neck back,the other groups were treated with D-galactose by continuously subcutaneous injection at the neck back to establish the subacute aging model. During the modeling,the rats in the drug treatment groups were treated with vitamin E,Liuwei Dihuang Pill and Zuogui Drink with high-and low-dose by intragastric administration,respectively,and the control and model group were treated with distilled water at equal volume. After administration for continuous 8 weeks,the liver and kidney tissue of rats were collected and made into the pathological sections for microscopic observation after HE staining. The positive expression levels of apoptosis related proteins（ Bax,Bcl-2 and Caspase-3） in the liver and kidney tissue were detected by immunohistochemistry,and the IOD values were analyzed and compared. Results （1）Compared with the control group,there were obvious pathological injuries of liver and kidney tissue in the model group. Compared with the model group,Zuogui Drink could alleviate the pathological damage of liver and kidney tissue in the aging process induced by D-galactose in rats.（2）Compared with the control group,the IOD values of Bax and Caspase-3 in the liver were significantly increased in the model group（ P 0. 01）,and the IOD value of Bcl-2 was significantly decreased（ P 0. 01）. Compared with the model group,the IOD values of Bax and Caspase-3 in the liver were significantly decreased in the Zuogui Drink group with high-dose（ P 0. 01）,and the IOD value of Bcl-2 wa

关 键 词：左归饮 补益肾阴 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]