大鼠弥漫性脑损伤Caspase-3表达及细胞凋亡的检测  

Detection of Caspase-3 Expression and Apoptosis in Diffuse Brain Injury in Rats

作　　者：李金星[1,2] 赵海梅 张方成[4] LI Jinxing;ZHAO Haimei;ZHANG Fangcheng(Department of Neurvsurgery, The Third Affiliated Hospital of Nanehang University, Nanehang Jiangxi 330008, China;Department of Neurosurgery, Hospital of Traditional Chinese Medicine of Shenzhen City, Shenzhen Guangdong 518033, China;Department of Geriatrics, The Third Affiliated Hospital of Nanchang University, Nanchang Jiangxi 330008, China;Department of Neurosurgery, Union Hospital, Huazhong University of Science and Technology, Wuhan Hubei 430001, China)

机构地区：[1]南昌大学第三附属医院神经外科,江西南昌330008 [2]深圳市中医院神经外科,广东深圳518033 [3]南昌大学第三附属医院老年医学科,江西南昌330008 [4]华中科技大学附属协和医院神经外科,湖北武汉430001

出　　处：《中国继续医学教育》2017年第26期136-138,共3页China Continuing Medical Education

基　　金：江西省科技厅科技支撑计划(赣科发计字2007-200/k1);江西省南昌市科技局社会发展计划(洪财企2008-68号)

摘　　要：目的研究大鼠弥漫性脑损伤脑细胞凋亡及Caspase-3表达的时程变化。方法参照Mamarou方法制作大鼠弥漫性脑损伤模型,免疫组化法检测Caspase-3蛋白在损伤脑组织中的分布,流式细胞术检测细胞凋亡率,透射电镜观察凋亡细胞的超微结构。结果弥漫性脑损伤后3 h皮层细胞Caspase-3表达增高,72 h达高峰,持续高表达至7 d。流式细胞术检测显示细胞凋亡在损伤后3 h增加,24~72 h为高峰期。结论弥漫性脑损伤后皮层Caspase-3表达增高,细胞凋亡率增高,细胞凋亡参与了脑损伤后的继发病理损害过程。Objective To study the timing changes of Caspase-3 expression and cell apoptosis of cerebral cortex in diffuse brain injury of rats. Methods Mamarou, free falling body method was used to induce diffuse brain injury of rats. Caspase-3 expression in injury Cerebral cortex was determined by immunohistochemistry immunostaining. Cell apoptosis rate was detected by fluorescence cellar methods, and ultrastructure of apoptosis cell was observed through electron microscope. Results Immunohistochemistry immunostaining showed that Caspase-3 expression rapidly upregulated 3 h after trauma and reach its peak at 72 h, and kept high level till 7 days. Cell apoptosis rate increased 3 h after trauma, the peak period was 24~72 h. Conclusion Caspase-3 expression and cell apoptosis rate increase in diffuse brain injury after trauma. Cell apoptosis contribute to the secondary pathological cascades of diffuse brain injury.

关 键 词：弥漫性脑损伤 凋亡 CASPASE-3 大鼠 

分 类 号：R651.15[医药卫生—外科学]