谷氨酸对雄性大鼠下丘脑GnRH基因表达的影响  

Effect of glutamate on hypothalamic GnRH gene expression in male rats

作　　者：阎彬彬[1] 任淑军[2] 杨军[3] 吕春梅[1] 曹兴福[1] 张雅娟[2] 夏蕾[2] 

机构地区：[1]哈尔滨医科大学生理学教研室,黑龙江哈尔滨150086 [2]哈尔滨医科大学第一临床医学院计划生育研究所,黑龙江哈尔滨150001 [3]绥棱农场医院,黑龙江绥棱152200

出　　处：《哈尔滨医科大学学报》2002年第4期270-272,共3页Journal of Harbin Medical University

基　　金：黑龙江省科委基金资助项目 (G92Z -2 2 -5 4)

摘　　要：目的　观察脑室注射谷氨酸 (Glu)对成年雄性大鼠下丘脑内侧基底部 (MBH)促性腺激素释放激素 (GnRH)及其mRNA表达的影响。方法　脑室注射Glu ,对GnRH的含量进行放射免疫测定 (RIA) ,用逆转录聚合酶链反应(RT PCR)方法观察GnRHmRNA的含量。结果　脑室注射 40 μmol L的Glu 2 0 μl,大鼠下丘脑GnRH含量增加 ,GnRHmRNA水平升高 ,Glu的NMDA受体拮抗剂MK 80 1可阻断此作用。结论　Glu可能通过提高mRNA的稳定性促进GnRH的基因表达 ,使下丘脑GnRH的含量增高 。Objective To study effect of intracerebroventricular injection of glutamate (Glu) on hypothalamic gonadotrophin-releasing hormone (GnRH) content and mRNA expression in adult male rats.Methods The GnRH content was studied by RIA and the GnRH mRNA level was measured by reverse transcriptase-polymerase chain reaction (RT-RCR). Results After intracerebroventricular injection Glu 20μl(40μmol/L), the GnRH content and mRNA level in experimental groups were significantly higher than in control ones. The e- ffect could be blocked by MK-801 (special NMDA-R antagonist).Conclusion Glu could enhance GnRH mRNA stability, increasing hypothalamic GnRH content and this action may be mediated by NMDA-R.

关 键 词：GNRH 基因表达 谷氨酸 促性腺激素释放激素 下丘脑 大鼠 放射免疫法 

分 类 号：R335[医药卫生—人体生理学]