体外受精子代小鼠性成熟期心血管功能的评估  

Effects of in vitro fertilization on the cardiovascular function of adult offspring mice

作　　者：张婷 王菁 崔毓桂 严正杰 高莉 刘嘉茵 

机构地区：[1]南京医科大学第一附属医院生殖医学科生殖医学国家重点实验室,210029

出　　处：《中华生殖与避孕杂志》2017年第10期803-807,共5页Chinese Journal of Reproduction and Contraception

基　　金：国家自然科学基金(81401267);国家重点基础研究发展规划(973计划)(2012CB944902);卫生部行业科研专项(201402004)~~

摘　　要：目的探讨体外受精(IVF)子代心血管系统的安全性。方法利用C57BL/6J小鼠建立IVF子代小鼠模型(IVF组,n=12),以C57BL/6J自然妊娠子代小鼠为对照组(n=12),观察比较小鼠性成熟期的体质量、血压,并利用彩色超声评估其心脏结构及功能。结果 12周龄IVF组子代小鼠的体质量[(26.13±4.58)g]高于对照组[(22.50±2.37)g],差异有统计学意义(P<0.05);仔鼠的血压组间无统计学差异(P>0.05)。彩色超声显示仔鼠心血管功能的主要指标(对照组,IVF组),左心室射血分数(65.41%±5.26%,65.77%±6.71%)、左室短轴缩短率(35.41%±4.05%,35.81%±4.84%)、颈动脉中内膜厚度[(0.05±0.01)mm,(0.05±0.01)mm]等无统计学差异(P>0.05);左房内径[(2.13±0.12)mm,(2.25±0.10)mm]、左心室质量指数[(8.24±0.46)g/m^2,(11.87±2.03)g/m^2]、主动脉内径[(1.33±0.06)mm,(1.45±0.11)mm]3项指标IVF组显著高于对照组(P<0.05)。结论 IVF子代小鼠与自然妊娠小鼠相比,部分心血管指标存在差异,提示IVF对性成熟期子代小鼠的心血管结构与功能可能存在潜在的影响,需要更多的远期随访。Objective To evaluate cardiovascular health in adult mice derived from an in vitro fertilization（IVF）procedure. Methods We examined the body weight and blood pressure in adult C57 BL/6 J mice（12 weeks） that were conceived by IVF and natural conception（NC）. Cardiovascular structure and function was evaluated by Doppler echocardiography. Results The body weight was significantly higher in IVF mice than NC mice [（26.13 ± 4.58） g,（22.50 ± 2.37） g]（P0. 05）. The blood pressure was similar in the two groups. The main parameters on cardiovascular structure and function were similar between NC and IVF groups（P0.05）, including left ventricular ejection fraction（65.41% ± 5.26%, 65.77% ± 6.71%）, left ventricular fractional shortening（35.41% ± 4.05%, 35.81% ± 4.84%）,and carotid intima-media thickness [（0.05 ± 0.01） mm,（0.05 ± 0.01） mm], whereas several other parameters, such as left atrial dimension [（2.13 ± 0.12） mm,（2.25 ± 0.10） mm], left ventricular mass index [（8.24 ± 0.46） g/m^2,（11.87 ±2.03） g/m^2] and aortic dimension [（1.33±0.06） mm,（1.45±0.11） mm], were significantly increased in IVF group compared with NC group（P0.05）. Conclusion Mice conceived by IVF manifested a statistically significant increase in several cardiovascular structure parameters. Our findings indicate that IVF procedure may make a potential effect on cardiovascular structure and function among IVF offspring, which calls for further investigation and longer-term follow-ups.

关 键 词：辅助生殖技术(ART) 子代 心血管 安全性 小鼠 

分 类 号：R714.8[医药卫生—妇产科学] R-332[医药卫生—临床医学]