苜蓿SSR引物在蚕豆上的通用性及SSR引物在F2群体中的偏分离分析  

Universality assessment of SSR primers from Medicago truncatula in fababean and their segregation distortion analysis in F_2 population

作　　者：田莹莹 侯万伟[2] 陈扣梅 武学霞 刘玉皎[2,3,4] 

机构地区：[1]青海大学,青海西宁810016 [2]青海大学农林科学院,青海西宁810016 [3]农业部作物基因资源与种质创制青海科学观测试验站,青海西宁810016 [4]省部共建三江源生态与高原农牧业国家重点实验室,青海西宁810016

出　　处：《青海大学学报（自然科学版）》2017年第6期1-5,共5页Journal of Qinghai University(Natural Science)

基　　金：基金项目:国家食用豆产业技术体系(CARS-09)

摘　　要：为了探究苜蓿SSR引物在蚕豆上的通用性及SSR引物在F2群体中的偏分离分析,本研究用216对苜蓿SSR引物研究在青海地区蚕豆的4个地方品的通用性,并且以云122与TF-42构建的F2群体为引物偏分离分析材料,共检测786对SSR引物。结果表明:苜蓿引物在蚕豆中的通用性比率为76.39%,多态性比率为6.02%;在亲本中具有多态性的引物为97对,26对引物在群体中具有多态性,在P<0.05显著水平上,共有19个分子标记表现偏分离,占总标记数目的比例为76%,其中有12个标记偏向父本TF-42,共占偏分离标记总数的比例为63.2%;3个标记偏向母本云122,共占偏分离标记总数的比例为15.8%;4个标记偏向杂合体,共占偏分离标记总数的比例为21%。蚕豆中引物偏分离比例较高,一定程度上有利于在链锁群中检测出偏分离热点区域。In this study, 216 SSR primers of M. Truncatula were tested for their transferability and polymorphism by PCR amplification with the genomic DNA of four fababean varieties. F2 materials derived from a cross between Yun122 x TF-42 were used as mapping population in faba bean. The results indicated that the transferability rate of M. Truncatula SSR in fababean was 76. 3 9 % , and the ratio of polymorphism SSR primers in the crop was 6. 0 2 % . Among the 97 polymorphic markers analyzed, 19 markers （ 7 6 % ） showed the genetic distortion （ P 〈0.05 ） in the F2 population. A -mong these segregation distortion SSR markers, 12 SSR marker alleles were distorted to the male parent TF-42 （ 63. 2% ）, 3 SSR markers alleles were distorted to the female parent Yun122 （15. 8% ） , and 4 SSR marker alleles were distorted to the heterozygote （21% ） . The higher rate of segregation distortion of SSR primers in the fababean,which may to some extent be good for the de-tection of segregation distortion regions （SDRs） in the linkage groups.

关 键 词：SSR 通用性 偏分离 

分 类 号：S529[农业科学—作物学]