梅花鹿鹿茸组织PDGFA基因cDNA克隆及序列分析  

Cloning and Sequence Analysis of the PDGFA Gene cDNA in Sika Deer Antler Tissue

作　　者：梁运涛 卢斌山 张强[1] 高悦禹 夏彦玲[1] 

机构地区：[1]东北林业大学,哈尔滨150040

出　　处：《东北林业大学学报》2017年第11期89-93,共5页Journal of Northeast Forestry University

基　　金：中央高校基本科研业务费专项资金项目(2572014CA01);中国博士后科学基金项目(20110491124)

摘　　要：为了探讨PDGFA基因的结构和功能,采用RT-PCR方法获得梅花鹿PDGFA基因,并运用生物信息学软件对其序列进行了分析。结果表明:梅花鹿PDGFA基因开放阅读框大小为591 bp,共编码196个氨基酸,蛋白的分子质量约为22 ku,理论等电点(pI)为8.53,是亲水性蛋白,具有信号肽。二级结构分析显示,含有47.45%无规则卷曲、39.29%α-螺旋、13.27%扩展链。进化分析显示,PDGFA蛋白与白尾鹿(Odocoileus virginianus texanus)、家牛(Bos taurus)、山羊(Capra hircus)、野猪(Sus scrofa)、白犀牛(Ceratotherium simum)、小鼠(Mus musculus)、密西西比鳄(Alligator mississippiensis)、原鸡(Gallus gallus)、绒啄木鸟(Picoides pubescens)的蛋白质序列相似度分别为98%、97%、96%、94%、93%、89%、81%、79%、78%。To explore the structure and function of PDGFA gene,we used the reverse transcriptase-polymerase chain reaction（ RT-PCR） to clone the cD NA sequence of the PDGFA gene from sika deer（ Cervus nippon hortulorum）. We further analyzed the sequence using different bioinformatics software. The open reading fragment size of the PDGFA gene was 591 bp,encoding 196 amino acids. The relative molecular weight of PDGFA protein was about 22 kD and the theoretical isoelectric point（ pI） was 8.53. PDGFA protein was hydrophilic and had a signal peptide. From secondary structure analysis,PDGFA protein included 47.45% random curl,39.29% alpha-helix and 13.27% extension chain. By evolutionary analysis,PDGFA protein sequences shared the homologies with Odocoileus virginianus texanus,Bos taurus,Capra hircus,Sus scrofa,Ceratotherium simum,Mus musculus,Alligator mississippiensis,Gallus gallus,Picoides pubescens were 98%,97%,96%,94%,93%,89%,81%,79% and 78%,respectively.

关 键 词：梅花鹿 PDGFA基因 克隆 序列分析 进化分析 

分 类 号：S825.2[农业科学—畜牧学]