一种含纳米银的物理交联壳聚糖-泊洛沙姆膜的制备与表征  

Preparation and characterization of a kind of physical crosslinking chitosan-poloxamer membrane containing nano-silver

作　　者：胡云睿 李达恒 陈嘉 余学飞[2] 奚廷斐 罗晓梅[7] 陈坤 

机构地区：[1]南方医科大学药学院广东省新药筛选重点实验室,广州510515 [2]南方医科大学生物医学工程学院,广州510515 [3]广州南沙资讯科技园有限公司博士后科研工作站,广州511458 [4]广州肽然生物科技有限公司,广州511458 [5]北京大学前沿交叉学科研究院生物医用材料与组织工程中心,北京100871 [6]北京大学深圳研究院,广东深圳518055 [7]广东银研高新材料股份有限公司,广州511458

出　　处：《功能材料》2017年第11期11001-11006,共6页Journal of Functional Materials

基　　金：国家级大学生创新创业资助项目(201512121017);广州市高校创新创业教育资助项目(2017074208);南方医科大学大学生创新创业资助项目(201512121198);2017"攀登计划"广东大学生科技培育专项资金科技发明制作资助项目(PDJH201760101)

摘　　要：为了简单方便地制备出一种柔韧性、吸水能力较好的,并具有一定抑菌特性的薄膜,通过调节溶解在稀醋酸溶液中的壳聚糖-泊洛沙姆溶胶pH值的方法,制备出了具有优良抑菌特性、柔韧性与吸水特性的薄膜。薄膜通过物理交联法制备,并进行了力学测试、薄膜断面SEM测试、吸水能力测试以及抑菌圈试验。测试结果显示,所制备的薄膜的拉伸强度和断裂伸长率与泊洛沙姆和甘油的浓度有关。在薄膜的断面SEM测试中发现,泊洛沙姆的加入,会使凝胶断面出现孔洞,孔洞的最大直径<10μm。在吸水能力测试中发现,在PBS溶液中,4‰与6‰泊洛沙姆组和2%甘油组具有更好地吸水能力;而在生理盐水溶液中,8‰泊洛沙姆组和2%甘油组具有更好的吸水能力。在抑菌圈试验中,发现不同泊洛沙姆浓度制备出的薄膜基本上均具有明显的抑菌圈。最后,通过综合以上特性,优选出泊洛沙姆和甘油的浓度分别为6‰和2%。由于其制备的方便性、薄膜较好的柔韧性、吸水性,以及有效的抑菌特性,壳聚糖-泊洛沙姆薄膜有望在烧伤治疗、伤口化脓性感染的治疗方面得以应用。In order to prepare a kind of membrane which has a certain antibacterial property,well flexibility and good water absorption capacity,in this study,such a kind of membrane was prepared by the method of adjusting pH value of chitosan-poloxamer sol which were dissolved in dilute acetic acid solution.Membrane had been prepared by physical crosslinking method.Mechanical test,SEM test of the membranes’ cross-section,absorptive capability and inhibition zone test had been carried out to test membrane’s properties.The test results showed that the tensile strength and the elongation at break of prepared membrane were related to the concentration of poloxamer and glycerol.In SEM test of the cross-sectional of chitosan-poloxamer membrane,it had been found that the addition of poloxamer will induce the appearance of holes and the maximum diameter of the hole was less than 10μm.In the water-absorbing ability test,it had been found that 4‰ and 6‰ poloxamer group and 2% glycerol group had better water absorption capacity in PBS solution,while 8‰ poloxamer group and 2% glycerol group had better water absorption capacity in physiological saline solution.In the zone of inhibition test,it had been found that prepared membranes with different concentrations of poloxamer essentially had obvious inhibition zone.Finally,by combining the above properties,the optimal concentration of poloxamer and glycerol was 6‰ and 2%,respectively.Due to the convenience of preparation,good flexibility,good water absorption capacity and effective antibacterial properties,chitosan-poloxamer membrane was expected to be applied in the treatment of burns and purulent wound infections.

关 键 词：壳聚糖-泊洛沙姆薄膜 纳米银 表征 

分 类 号：R318.08[医药卫生—生物医学工程]