不同养殖场家蝇成虫体表携带细菌的分离与鉴定  

Bacteria on body surface of Musca domestica adults from different livestock farms

作　　者：陈丹[1] 庄桂芬[1] 黄振东[1] 薛志静 刘婧[1] 李妍[1] 张瑞玲[1,2] 张忠[1,2] 

机构地区：[1]泰山医学院新发传染病溯源及防控协同创新中心,泰安271016 [2]泰山医学院基础医学院,泰安271016

出　　处：《中国人兽共患病学报》2017年第11期951-955,共5页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(Nos.81401693;81572028;81271874);山东省科技发展计划项目(No.2014GSF121007)联合资助~~

摘　　要：目的研究不同养殖场内家蝇成虫体表携带细菌数量和类别。方法采用网捕法对不同养殖场的家蝇进行采样,利用振荡洗脱法分离其体表细菌,对获得细菌的16SrDNA基因扩增后进行分离分子鉴定。结果不同养殖场内家蝇密度、体表携带细菌数量和种类有统计学差异,且家蝇密度与体表携带细菌数量和携带的细菌属数呈线性相关关系。在养殖场内分离到的家蝇体表携带细菌中,葡萄球菌属、克雷伯氏菌属、埃希菌属、沙雷氏菌属分布较广,同时分离的细菌中多为条件致病菌,存在疾病传播风险。结论养殖场家蝇密度反映出养殖场的整体管理水平;养殖场孳生的家蝇可机械性携带条件致病菌,存在将细菌传播给人类的风险,需要进一步加强对养殖场蝇类防控及携带细菌的监控。This study aims to isolate and identify the bacteria from the surface of Muscadomesticaadults in different livestock farms.The adults of Musca domestica were collected with sweep nets,then the surface of adult flies were washed with sterile water,and the eluted bacteria cultured with LB culture medium.The DNA of the bacteria isolated was extracted,16 SrDNA fragment was amplified through general PCR,and the 16 SrDNA sequence was blasted with bacteria sequence in NCBI.Results showed that the number of Musca domestica adults and the bacteria from their surface had significant different in different livestock farms.The population density of Musca domestica adults had linear correlation with the number of bacteria from Musca domestica adults and the number of bacteria genus.Among the bacteria isolated from Musca domestica adults,Staphylococcus,Klebsiella,Escherichiaand Serratia were the most common genus in the livestock farms.Most of the bacteria isolated from livestock farm house flies were opportunistic human bacterial pathogen,and the risk of bacterial pathogens spread to people was existed.The population density of house flies reflected the management level of different livestock farms.The house flies could mechanical transmit opportunistic human bacterial pathogen from livestock residue to human.So the house flies control and the monitoring of bacteria on body surface of house flies carried should be strengthened.

关 键 词：家蝇 养殖场 体表细菌 机会致病细菌 蝇传疾病 

分 类 号：R384[医药卫生—医学寄生虫学]