应用荧光PCR诊断鸡腺胃型传染性支气管炎  

Diagnosis proventriculus type infections bronchitis by applying real-time fluorescent quantitative PCR

作　　者：陈秀琴 黄梅清[1,2] 蔡羲 江斌[1,2] 陈少莺 吴南洋[1,2] 

机构地区：[1]福建省农业科学院畜牧兽医研究所,福州350013 [2]福建省畜禽疫病防治工程技术研究中心,福州350013

出　　处：《福建畜牧兽医》2017年第6期10-12,共3页Fujian Journal of Animal Husbandry and Veterinary medicine

基　　金：福建省现代农业鸡产业技术体系(2013-2017年)资助

摘　　要：应用荧光PCR试剂盒对临床表现为消瘦和腺胃肿大为特征的患鸡病料进行鸡传染性支气管炎病毒(IBV)、禽网状内皮组织增殖病病毒(REV)和鸡马立克氏病病毒(MDV)核酸检测。结果显示:鸡传染性支气管炎病毒荧光RT-PCR检测为阳性,禽网状内皮组织增殖病病毒荧光RT-PCR检测为阴性,鸡马立克氏病病毒荧光PCR检测为阴性。结合患鸡精神沉郁,极度消瘦,腺胃肿大如球状等的临床表现,综合分析表明该患鸡发病主要病因为感染鸡腺胃型传染性支气管炎病毒。The study detected the nucleic acid of diseased chickens with clinical symptoms of emaciation and swollen proventriculusby using fluorescent PCR kit. The detection including infections bronchitis virus （IBV）, Reticuloendotheliosis virus （REV）and Marek＇sDisease （MDV）. The results showed that IBV was positive but REV and MDV were negative. Combing the clinical symptoms of actingdepressed, extreme emaciation and swollen proventriculus, thus, it was concluded that the diseased chickens were infected with IBV.

关 键 词：传染性支气管炎 腺胃型 病原检测 

分 类 号：S858.31[农业科学—临床兽医学]