电化学发光分析法测定糖尿病相关二肽  

An Electrochemiluminescent Method to Detect A Dipeptide Related to Diabetes

作　　者：方晨 郑丽娟 翟苏燕 屠一锋[1] 

机构地区：[1]苏州大学材料与化学化工学部,苏州215123 [2]苏州大学第二附属医院,苏州215004

出　　处：《分析化学》2017年第12期1951-1955,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.21375091)资助~~

摘　　要：将纳米金粒子负载于氧化铟锡导电玻璃(ITO)表面作为电化学发光(ECL)工作电极,建立了简单、快速测定二肽的方法。采用透射电镜、扫描电镜、电化学和光谱等技术方法表征材料和所制备电极的性能。在最优条件下,组氨酸-丙氨酸二肽分子(His-Ala)对鲁米诺在此电极上的ECL有显著的猝灭作用,从而可以对二肽进行检测。在2.44×10^(-11)~1.22×10^(-7)mol/L浓度范围内,ECL响应和二肽浓度有良好的线性关系,检出限为2.42×10^(-12)mol/L(S/N=3)。人体内胰高血糖素样肽-1(GLP-1)在二肽基肽酶Ⅳ(PDD-Ⅳ)作用下失去活性并释放出同量的His-Ala二肽,因此,本方法可通过测定血液中二肽浓度间接测定GLP-1,以及评估PDD-Ⅳ和其抑制剂的活性。上述DPP-Ⅳ抑制剂可调节2型糖尿病人血液GLP-1在正常水平,是糖尿病研究和治疗中具有潜在价值的药物设计靶点。In this work,a simple and fast approach for dipeptide detection was developed. Au NPs were decorated onto the surface of indium tin oxide( ITO) glass to serve as the working electrode to trigger the electrochemiluminescence( ECL) of luminol. The property of this electrode was characterized by transmission electron microscopy( TEM), scanning electron microscopy( SEM), electrochemistry and spectroscopic method. Under the optimum conditions,the dipeptide His-Ala could be detected within a linear range from2. 44×10(-11) mol/L to 1. 22 × 10^(-7) mol/L,with a detection limit of 2. 42 × 10^(-12) mol/L( S/N = 3). In human body,the glucagon-like peptide 1( GLP-1) might lose activity during degradation under the enzymatic action of dipeptidyl peptidase Ⅳ( PDD-Ⅳ),meanwhile release same concentrated His-Ala dipeptide. Thus,the detection of His-Ala dipeptide was of great significance for investigation of diabetes not only for understanding the relation between GLP-1,PDD-Ⅳ and its inhibitor,but also the drug discovering because of its potential availability as the target to control the blood GLP-1 level of type II diabetics.

关 键 词：电化学发光 鲁米诺 糖尿病 胰高血糖素样肽-1 二肽 二肽基肽酶IV 

分 类 号：O657.1[理学—分析化学] R587.1[理学—化学]