硅硼掺杂碳点的制备及其在血红蛋白传感中的应用  

Preparation of Silicon and Boron-doped Carbon Dots and Their Application in Hemoglobin Sensing

作　　者：杨婷[1] 周影 汪宁 陈明丽[1] 王建华 

机构地区：[1]东北大学理学院化学系分析科学研究中心

出　　处：《分析化学》2017年第12期1996-2003,共8页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.21235001;21405010;21675019;21375013);中央高校基本科研业务费(Nos.N160504008;N141008001);电分析化学国家重点实验室开放课题(No.SKLEAC201702)项目资助~~

摘　　要：杂原子掺杂是提高碳点荧光性能的有效手段。本研究以柠檬酸(C_6H_8O_7)、硼酸(H_3BO_3)、3-氨丙基三乙氧基硅烷(APTES)为原料,采用微波法一步制备硅和硼掺杂的碳点(Si BCDs);在Si BCDs前驱体中加入聚丙烯酸钠(PAAS),微波法制备了水溶性好、量子产率高的PAAS-Si BCDs。采用X射线衍射(XRD)、X射线光电子能谱(XPS)及红外光谱(FT-IR)对制备的碳点进行了表征。SiBCDs粒径约4~8 nm,PAAS-Si BCDs平均粒径5.2 nm,两者最大激发波长和发射波长分别为350和445 nm,荧光量子产率(QY)分别为20.1%和34.6%。基于血红蛋白对PAAS-Si BCDs的荧光猝灭效应,建立了全血样品中血红蛋白(Hb)的检测方法,线性范围为0.21~5.22μmol/L,检出限为0.06μmol/L(S/N=3)。Heteroatom doping is an effective way to elevate the fluorescent performance of carbon dots. In this study,a microwave one-pot approach for the synthesis of Si BCDs was proposed by using citric acid( C_6H_8O_7),boric acid( H_3BO_3) and( 3-aminopropyl) triethoxy sliane( APTES) as source materials. PAASSi BCDs were prepared with the assistance of microwave when sodium polyacrylate( PAAS) was added in the precursor of Si BCDs. The products were characterized by X-ray diffraction( XRD),X-ray photoelectron spectroscopy( XPS) and Fourier transform infrared( FT-IR) spectra,respectively,confirming that the product was amorphous CDs,with small amount of Si and B. The synthesized Si BCDs had good monodispersity with size of 4-8 nm,the average size of PAAS-Si BCDs was 5. 2 nm,and the excitation/emission maximum was 350 nm/445 nm. The quantum yield of Si BCDs was 20.1%,and that of PAAS-Si BCDs was 34.6%. Based on the quenching effect of hemoglobin( Hb) on the fluorescence PAAS-Si BCDs,a sensitive Hb detection method was developed. A linear range of 0.21-5.22 μmol/L was obtained together with a detection limit of 0.06 μmol/L. The method was applied in the determination of Hb in human blood samples.

关 键 词：碳点 硅硼掺杂 血红蛋白 传感分析 

分 类 号：O657.3[理学—分析化学] Q-33[理学—化学]