蒙山黑土猪及其种群氟烷基因PCR-RFLP检测与分析  

Detection and Analysis of Halothane Gene in Mengshan Black Pig and Its Population with Using PCR-RFLP

作　　者：谢丽华[1] 戴光文 梁真[1] 唐国春 黄树新 黄超[1] 吴颖航 

机构地区：[1]梧州市动物疫病预防控制中心,广西梧州543002 [2]蒙山县动物疫病预防控制中心,梧州546700 [3]蒙山县强新牲猪专业合作社,梧州546700 [4]广西蒙山金宇特色农业发展有限公司,梧州546700

出　　处：《养猪》2017年第6期61-64,共4页Swine Production

基　　金：2015年梧州市科学研究与技术开发计划"黑土猪产业化新技术研发与示范"项目(201501025)

摘　　要：采用成熟的商品化PCR-RFLP试剂盒检测分析了蒙山黑土猪核心种群、2代种群及2~3代商品猪群的氟烷基因类型。结果显示,核心种群的陆川猪、杜洛克及2代种猪和商品猪均为显性纯合子Hal ~NHal ~N基因型,核心野猪种群有1头检测为杂合子Hal ~NHal^n基因型,全猪群无隐性纯合子Hal^(n )Hal^n基因型。野猪群和全猪群Hal^n等位基因频率分别为0.033和0.005。结果表明,商品化PCR-RFLP试剂盒可快速准确诊断不同类型猪种的氟烷基因类型,蒙山黑土猪及其种群不表现氟烷敏感临床特征,Hal ~NHal ~N基因型猪遗传性能稳定。The genotype of halothane（Hal） gene in Mengshan black pig and its population were analysized byusing commercial PCR-RFLP Kit. The core population included Lu Chuan pig, Duroc and the second generationpopulation and commercial pig were homozygous dominant genotype HalNHalN, a wild boar in core populationwas heterozygous genotype HalNHaln, the whole herd had no homozygous recessive genotype HalnHaln. The allelefrequencies Halnin wild boar population and all pigs were 0.033 and 0.005. The result showed that it can berapid and accurate diagnosis of the different types of halothane gene by using commercial PCR -RFLP Kit,Mengshan black pig and its population does not exhibit halothane-sensitive features, the genetic performance ofHalNHalN genotype pig is stable.

关 键 词：蒙山黑土猪 氟烷基因 PCR-RFLP 

分 类 号：S828[农业科学—畜牧学]