市售夏黑无核葡萄果表微生物多样性研究  

Study on the microbial diversity on the grape surface of marketed Summer Black Seedless variety

作　　者：张俊杰[1,2,3] 郭晨 尚益民[1] 杨旭 陈锦永[2] 张文叶[1] 刘崇怀[2] 

机构地区：[1]郑州轻工业学院食品与生物工程学院,河南郑州450001 [2]中国农业科学院郑州果树研究所,河南郑州450009 [3]食品生产与安全河南省协同创新中心,河南郑州450001

出　　处：《轻工学报》2017年第6期20-26,共7页Journal of Light Industry

基　　金：河南省自然科学基金项目(162300410330);郑州轻工业学院博士科研启动基金项目(2014BSJJ006)

摘　　要：运用宏基因组高通量测序技术对市售夏黑无核葡萄表皮菌群16S rRNA基因V3-V4可变区进行测序和分析,发现该葡萄表皮的细菌群落具有高度多样性,且优势菌群明显.从门水平上分析,群落主要为厚壁菌门和变形菌门;从纲水平上分析,群落主要为α,β,γ变形菌纲、梭菌纲和放线菌纲;从属水平上分析,乳酸球菌属为最优菌属.同时从葡萄表皮检出了Escherichia-Shigella(埃希氏菌属-志贺氏菌属)的致病菌群体.该研究成果对于果蔬采后生物防治和市售果蔬的检验检疫工作有一定的借鉴意义.Using the metagenomic high-throughput sequencing technology,the sequencing analysis of the V3-V4 variable regions of 16 S rRNA gene of the grape surface microbe of marketed Summer Black Seedless variety was conducted. The results showed that the the grape surface microbes were highly diverse consortium and had obviously dominant microflora. At the phylum level,the dominant microbes were Firmicutes and Proteobacteria. At the class level,the dominant microbes were α,β,γ-Mycetozoan,Clostridium and Actinomycetes. At the genus level,the dominant microbe was Lactococcus. Furthermore,the pathogenic bacteria EscherichiaShigella was detected on the grape surface of marketed Summer Black Seedless variety. This study is of reference significance for biological control after fruit picking and inspectation and quarrantine of market fruit.

关 键 词：夏黑无核葡萄表皮 微生物多样性 高通量测序病原菌 

分 类 号：TS255.2[轻工技术与工程—农产品加工及贮藏工程]