Double-lipase Catalyzed Synthesis of Kojic Dipalmitate in Organic Solvents  

作　　者：WANG Zhiyuan LUO Wen FU Junying LI Zhibing WANG Zhongming LU Pengmei 

机构地区：[1]Key Laboratory of Renewable Energy of Chinese Academy of Sciences, Guangdong Key Laboratory of New and Renewable Energy Research and Development, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, P. R. China

出　　处：《Chemical Research in Chinese Universities》2017年第6期903-907,共5页高等学校化学研究（英文版）

摘　　要：Kojic dipalmitate（KDP） was synthesized by the way of esterification ofpalmitic and kojic acids in organic solvent, with double-lipase as the biocatalyst. Four commercially available lipases（Amano PS, Novozym 435, Lipozyme TL IM and Lipozyme RM IM） were used to group six double-lipase combinations. These combinations were studied and Amano PS-Novozym 435 was found to have the best efficiency and was selected for optimizing the reaction conditions. The optimal reaction conditions were as follows. The mass ratio ofAmano PS to Novozyme 435 was 1：1.5, with stirring speed of 500 r/min, substrates molar ratio of 1：2, 50 ℃, 5%（mass fraction） catalyst dosage of kojic acid quantity and using acetone as co-solvent. Under these conditions, the diesterification of C5 and C7 hydroxyl groups of kojic acid molecule could be well catalyzed by double-lipase and realize a high KDP yield of 85%.Kojic dipalmitate（KDP） was synthesized by the way of esterification ofpalmitic and kojic acids in organic solvent, with double-lipase as the biocatalyst. Four commercially available lipases（Amano PS, Novozym 435, Lipozyme TL IM and Lipozyme RM IM） were used to group six double-lipase combinations. These combinations were studied and Amano PS-Novozym 435 was found to have the best efficiency and was selected for optimizing the reaction conditions. The optimal reaction conditions were as follows. The mass ratio ofAmano PS to Novozyme 435 was 1：1.5, with stirring speed of 500 r/min, substrates molar ratio of 1：2, 50 ℃, 5%（mass fraction） catalyst dosage of kojic acid quantity and using acetone as co-solvent. Under these conditions, the diesterification of C5 and C7 hydroxyl groups of kojic acid molecule could be well catalyzed by double-lipase and realize a high KDP yield of 85%.

关 键 词：Kojic acid Palmitic acid Kojic dipalmitate Double-lipase catalysis Diesterification 

分 类 号：O6[理学—化学]