脑内移植RMNE6细胞治疗缺血性脑损伤的效果  

Effects of intracerebral transplantation of RMNE6 on the ischemic injury of the brain

作　　者：武赟 翟晓艳[1] 杨李旺 徐群渊 

机构地区：[1]山西中医药大学基础医学院解剖学教研室,山西晋中030619 [2]首都医科大学神经生物学系,北京100069

出　　处：《解剖学报》2017年第6期635-641,共7页Acta Anatomica Sinica

基　　金：国家自然科学基金(81070977);山西省卫生计生委科研课题(20160113);山西中医学院博士科研启动基金(2016BK01)

摘　　要：目的为细胞替代治疗缺血性脑损伤选择合适的干细胞。方法将RMNE6细胞植入正常脑和缺血模型大鼠脑(8只)内,通过免疫荧光染色、行为学实验、影像学等方法观察其在脑内的状况,与安慰剂组(8只)及成纤维细胞移植组(8只)比较,观察其对模型动物梗塞体积的影响和模型动物行为学的改善。结果细胞移植后,RMNE6细胞在正常脑和缺血脑内可以存活,但移植有RMNE6细胞的缺血脑内出现肿瘤。细胞移植与否对梗死体积没有影响。细胞移植后,模型动物的行为学也未见明显改善。结论从安全性的角度考虑,RMNE6细胞不适合通过脑内移植治疗缺血性脑损伤,RMNE6细胞还需要改造。Objective To select the suitable stem cells for cell replacement in stroke therapy. Methods The RMNE6 cells were transplanted into the normal brain and injured brain of the stroke model of rats （ n = 8 ）. The fates of the RMNE6 cells in the normal brain and the injured brain, the effects on the ischemic volume and the improvements on the behavior of the rats were observed by immunofluorescenee staining, behaviour testing and magnetic resonance imaging, compared with the placebo group （n = 8） and the fibroblast group （n = 8）. Results After transplantation, the RMNE6 cells were able to survive in the normal and ischemic brain area. There was tumourigenesis in the ischemic brain transplanted with the RMNE6 cells. The cell transplantation had no effects on the size of the isehemic volume. The behavior of the model animals had no significiant improvement. Conclusion As far as the safety is concerned, the RMNE6 cells are not suitable for cell replacement in the stroke therapy and still need to be modified.

关 键 词：RMNE6细胞 细胞移植 脑缺血 免疫荧光 磁共振成像 大鼠 

分 类 号：Q189[生物学—神经生物学]