百合水溶液对麻黄素致损伤小鼠肺组织的影响  

Effects of lilium soup on lung histological structure in mice injured by ephedrine

作　　者：李重阳[1] 彭静[2] 俞诗源[2] 

机构地区：[1]甘肃政法学院公安分院,兰州730070 [2]西北师范大学生命科学学院,兰州730070

出　　处：《解剖学报》2017年第6期746-752,共7页Acta Anatomica Sinica

基　　金：甘肃省自然科学基金(1107RJZA141);兰州市社会发展项目(2013-3-72;2014-1-102)

摘　　要：目的探讨百合水溶液对麻黄素致损伤肺组织的影响。方法选出生10 d的小鼠72只,随机分为空白对照组、实验对照组和百合组3组。实验对照组和百合组采用递增剂量连续(1~5d,5~10d,11~15 d)腹腔注射(2.0、4.0、6.0 g/L)麻黄素溶液15 d(每次0.2 ml,每天两次),百合组注射麻黄素1 h后,再灌胃0.2 ml百合水溶液,实验对照组灌胃等量的生理盐水,空白对照组腹腔注射和灌胃等量的生理盐水。用比色法检测肺组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活性及血浆髓过氧化物酶(MPO)活性的变化,用石蜡包埋法和HE染色法观察小鼠肺组织结构的病理变化,采用免疫组织化学法检测小鼠肺组织Bax、核因子-κB(NF-κB)蛋白表达的变化。结果实验对照组肺组织SOD、CAT和GSH-Px活性显著降低(P<0.01或P<0.05),血浆MPO活性显著升高(P<0.01或P<0.05),肺组织出现明显病理性损伤,肺泡上皮细胞局部降解、脱落,肺泡直径和肺泡隔厚度增大(P<0.01),肺组织Bax、NF-κB蛋白的阳性表达明显增强(P<0.01或P<0.05)。与实验对照组相比较,百合组肺组织SOD、CAT和GSH-Px活性升高(P<0.01或P<0.05),血浆MPO活性显著降低(P<0.05),肺组织损伤明显改善,肺泡上皮脱落减少,肺泡直径和肺泡隔厚度均减小(P<0.01或P<0.05),肺组织Bax、NF-κB蛋白的阳性表达明显减弱(P<0.01或P<0.05)。结论百合水溶液能增强机体抗氧化能力,提高机体免疫力,抑制肺组织Bax、NF-κB蛋白的阳性表达和肺组织细胞凋亡,对麻黄素致损伤肺组织有一定的保护作用。Objective To explore the effects of lilium soup on ephedrine-injured mice lung. Methods Totally 72 Kunming mice ten days after birth were used and randomly divided into 3 groups：nature control group, experimental control group and lilium group. Experimental control group and lilium group were intraperitoneally injected escalation doses （2.0 g/L,4.0 g/L,6.0 g/L） ephedrine 0.2 ml twice every day. And nature control group was injected the same amount of saline. One hour after injection of ephedrine, lilium group was intragastric administration with 0.2 ml lilium soup. Experimental control group and nature control group were intragastric administed with the same amount of saline. The change of superoxide dismutase （SOD） , hydrogen peroxidase （CAT） and glntathione peroxidase （GSH-Px） activity of lung and the activity of plasma myeloperoxidase （MPO） were determined by colorimetry. HE staining was used to observe the change of histological structure of lung. Immunohistochemistry was used to detect the change of Bax and nuclear factor-kappa B （ NF- KB） expression in lung. Results The activities of SOD,CAT and GSH-Px of experimental control group were significantly lower than that of the nature control group （ P 〈 0.01 or P 〈 0.05 ） ~ The activity of plasma MPO of experimental control group were significantly increaser than that of the nature control group （ P 〈 0.01 or P 〈 0. 05 ）. Lung histopathologic damage of experimental control group was obvious. The alveolar structure were disorder and epithelial cells were fall off. The alveolar diameter and thickness of the alveolar septum increased significantly than the nature control group （P 〈 0.01 ）. The stained color and average optical density of Bax and NF-xB increased significantly in experimental control group than that of the nature control group （ P 〈 O. O1 or P 〈 0.05 ）. Compared with experimental control group, the activities of SOD, CAT and GSH-Px of lilium group increased （ P 〈 0. 01 or P 〈 0.05 ）. Th

关 键 词：百合水溶液 麻黄素 肺 组织结构 抗氧化物酶 免疫组织化学 小鼠 

分 类 号：R338.21[医药卫生—人体生理学]