超声、微泡及重组腺病毒联合介导外源性基质细胞衍生因子1α转染急性心肌梗死大鼠心脏对非靶组织的影响  

The influence of the transfection of SDF-1α to the heart mediated by recombinant adenovirus, microbubbles andultrasound on non targeted tissues of rats with acute myocardial infarction

作　　者：苏高峰[1] 刘丽云[1] 穆玉明[1] 

机构地区：[1]新疆医科大学第一附属医院心脏超声诊断科,乌鲁木齐830011

出　　处：《中华超声影像学杂志》2017年第11期996-1000,共5页Chinese Journal of Ultrasonography

基　　金：国家自然科学基金（8146（1266）;新疆重大疾病医学重点实验室开放课题项目（SKLIB-XJMDR-2014-13）

摘　　要：目的评价超声、微泡及重组腺病毒联合介导外源性基质细胞衍生因子1a（SDF-1a）基因转染急性心肌梗死（AMI）大鼠心脏时在非靶组织肝、肺及肾中的转染情况。方法成功建立AMI模型的40只SD大鼠完全随机分为4组：单纯超声辐照组（M＋U组／对照组，10只），超声辐照＋载pAd—EGFP／SDF-1a（生物素化的共表达绿色荧光蛋白和SDF-1a的重组腺病毒）基因微泡给药1d组（M＋S1＋U组，10只），连续给药2d组（M＋s2＋U组，10只）及连续给药3d组（M＋s3＋U组，10只）。术后7d，激光共聚焦显微镜观察非靶组织肝、肺及肾中绿色荧光蛋白的表达情况。结果对照组肝、肺及肾中均无绿色荧光蛋白表达，给药1d、2d、3d组肝、肺及肾中均有少量表达，给药组与对照组相比差异均具有统计学意义（P〈0．05），但给药组间差异无统计学意义。结论超声、微泡及重组腺病毒联合介导外源性SDF-1a基因转染AMI大鼠心脏的同时，非靶组织也会被转染，但随着给药天数增加，靶基因在非靶组织的转染只产生了轻微的累积，靶基因在非靶组织中的转染效果与给药天数并不呈正相关。Objective To evaluate gene transfection in liver, lung and kidney by ultrasound, microbubble and recombinant adenovirus mediated exogenous stromal cell derived faetor-la （SDF-la） gene transfer to the heart in rats with acute myocardial infarction（AMI）. Methods Forty AMI SD rats were randomly divided into control and experimental groups：myocardial infarction ＋ ultrasound irradiation group （M＋ U/control group, n = 1（I）, and 3 experimental subgroups on the basis of pAd-EGFP/SDF-la （The biotin recombinant adenovirus expressing enhanced green fluorescent protein and SDF-Ia）. Genes transfection length of time ： 1 day, 2 days and 3 days of transfection （ M ＋ S1 ＋ U, M ＋ $2 ＋ U and M ＋ S3 ＋ U group）. The expression of EGFP in liver, lung and kidney were detected by laser scanning confocal microscopy at 7 days after administration. Results There was a little expression of EGFP in the liver, lung and kidney in the drug delivery group and no expression in the control group. The differences in the expression of EGFP between all the gene delivery groups and the control group were statistically significant （ P 〈0.05）. With the increase of the number of medication days, the target gene transfection increased slightly, but there was no significant difference among the different drug delivery groups. Conclusions When the ultrasound, microbubble and recombinant adenovirus mediated exogenous SDF 1 a gene transfer to the heart in AMI rats, liver, lung and kidney tissues will also be transfected. However, with the increasing of the days of administration, the transfection of target gene in non-target tissue produces only a slightaccumulation. The transfection area of target gene in non-target tissue is not linear correlated with the days of administration.

关 键 词：超声靶向微泡破坏 急性心肌梗死 基质细胞衍生因子1Α 转染 

分 类 号：R542.22[医药卫生—心血管疾病] R544.1[医药卫生—内科学]