苦豆子总碱对自发性高血压大鼠动脉血压影响  

Mechanism of Total Alkaloid Sophora Alopecuroides on Reducing Arterial Blood Pressure in Spontaneously Hypertensive Rats

作　　者：牛彩琴[1] 雷枭[1] 张团笑[1] 

机构地区：[1]川北医学院,四川南充637007

出　　处：《辽宁中医药大学学报》2017年第11期32-35,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：四川省教育厅重点项目(08ZA109;14ZA0182)

摘　　要：目的 :探讨苦豆子总碱(total alkaloid of Sophora alopecuroides,TASA)降低自发性高血压大鼠(SHR)动脉血压的机制。方法 :(1)将42只16周龄的SHR随机分为SHR模型对照组、TASA组和5组拮抗剂(左旋硝基精氨酸、甲烯蓝、维拉帕米、普萘洛尔和吲哚美辛)组;另取8只同周龄Wistar大鼠作为正常对照组。TASA组:股静脉注射TASA(2、4和8 mg·kg^(-1));拮抗剂组:先分别注射对血压无影响的阻滞剂后,再给TASA(8 mg·kg^(-1));SHR组和正常组注射等量生理盐水。经颈总动脉插管记录各组给药前后的动脉血压。(2)颈动脉血压记录30 min以后,抽取正常组、SHR组和TASA组大鼠的血液2 mL,提取血清,以酶联免疫法测定各组NO、ET-1和AngⅡ的含量。结果 :(1)TASA能显著降低SHR的血压,并具有量效依赖性(SBP:r=0.83,t=56.52,P<0.01;DBP:r=0.78,t=45.92,P<0.01;MAP:r=0.83,t=54.43,P<0.01)。维拉帕米、左旋硝基精氨酸和甲烯蓝可明显抑制TASA(8 mg/kg)的降压效应(P<0.01);而吲哚美辛、普萘洛尔对TASA的作用无影响。(2)SHR组的ET-1和AngⅡ明显高于正常组,而NO低于正常组(P<0.05);TASA组的ET-1和AngⅡ显著低于SHR组,而NO则高于SHR组(P<0.05)。结论 :TASA降低SHR血压与其恢复血管内皮细胞功能释放NO的增多和减少细胞钙内流有关。Objective ：To research mechanism of total alkaloid Sophora alopecuroides（TASA）on reducing arterial blood pressure in spontaneously hypertensive rats（SHR）. Methods ：（1）42 SHR rats of 10 weeks old were randomly divided into the model group,TASA group and Antagonist groups. The antagonistic groups included Nω-nitro-L-arginine（L-NNA）,methylene blue（MB）,verapamil（Vpm）,propranolol（Pro）and indomethacin（Ind）group. 8 same week old Wistar rats were used as control group. TASA group was cumulative injected of TASA（2,4,and 8 mg·kg^（-1））in the femoral vein. Antagonists were separately injected to antagonist groups and until finded the appropriate dose of blockers which has no effect on blood pressure,then the antagonist groups were separately injected with TASA（8 mg·kg^（-1））;model group（SHR）and control group were given the same volume physiological salin. Carotid artery blood pressure of rats was recorded before and after administration through common carotid arterial cannula.（2）After recording blood pressure 30 minutes,2 mL blood samples of rats were separately collected in the normal group,SHR group and TASA group. Then the contents of NO,ET-1 and Ang II in each group extractions of serum were determined by enzyme linked immunosorbent assay. Results ：（1）TASA can significantly reduce the blood pressure of SHR and has a dose-effect dependence in vivo（SBP ：r=0.83,t=56.52,P0.01;DBP ：r=0.78,t=45.92,P0.01;MAP ：r=0.83,t=54.43,P0.01）. The effect of the decreasing arterial blood pressure of TASA（8 mg/kg）was significantly blocked by L-NNA,verapamil and MB,but there was no effect with indomethacin and propranolol.（2）ET-1 and Ang II in SHR group were significantly higher,while NO was lower than the normal group（P0.05）. ET-1 and Ang II in TASA group were significantly lower,while NO was higher than the model group（P0.05）. Conclusion ：TASA can decrease SHR blood pressure. The mechanism may be related with the increase of NO levels throung rep

关 键 词：苦豆子总碱 自发性高血压 颈总动脉 血压 一氧化氮 钙离子 

分 类 号：R285.5[医药卫生—中药学]