调脂Ⅰ号方对动脉粥样硬化大白兔血清炎症因子及腹主动脉LDL-R、VCAM-1mRNA表达的影响  

Effect of Tiaozhi Fang Ⅰ on Serum Inflammatory Factor and Expressions of Aorta Abdominalis LDL-R and VCAM-1mRNA in Atherosclerosis Model Rabbits

作　　者：刘中勇[1] 李林[1] 方家[1] 

机构地区：[1]江西中医药大学附属医院

出　　处：《中医杂志》2017年第23期2049-2053,共5页Journal of Traditional Chinese Medicine

基　　金：江西省科技计划(20135BBG70002)

摘　　要：目的探讨调脂Ⅰ号方抗动脉粥样硬化的可能作用机制。方法将48只雄性新西兰大白兔随机分为假手术组8只及中药高剂量组、中药低剂量组、阳性对照组、模型组各10只。除假手术组外,其余各组均饲以高脂饲料建立动脉粥样硬化模型。假手术组和模型组以10 ml/(kg·d)生理盐水灌胃。阳性对照组以30 mg/(kg·d)阿托伐他汀钙灌胃。中药高、低剂量组给予调脂Ⅰ号方12.10、3.02 g/(kg·d)灌胃。3个月后测定各组大鼠血清总胆固醇(TC)、甘油三脂(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、超氧化歧化酶(SOD)和丙二醛(MDA)含量及血清中肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、细胞间黏附分子1(ICAM-1)、血管细胞黏附分子1(VCAM-1)的含量。RT-PCR法检测腹主动脉低密度脂蛋白胆固醇受体(LDL-R)、VCAM-1mRNA表达。结果与模型组比较,中药高、低剂量组TC、TG、LDL-C、MDA显著下降,SOD水平显著升高,中药高剂量组TNF-α、IL-6、ICAM-1、VCAM-1含量及VCAM-1mRNA表达显著降低,LDL-R mRNA表达显著升高(P<0.05或P<0.01)。与阳性对照组比较,中药高剂量组SOD含量显著升高,TNF-α水平显著降低(P<0.05)。中药高、低剂量组各指标比较差异均无统计学意义(P>0.05)。结论调脂Ⅰ号方可改善血脂、降低血清炎性因子,上调LDL-R mRNA,下调VCAM-1 mRNA,可能是其抗动脉粥样硬化的机制之一。Objective To explore the possible mechanism of Tiaozhi Fang Ⅰ against atherosclerosis. Methods Forty-eight male New Zealand white rabbits were randomized into sham operation group（ n = 8）,the high dose herb group（ n = 10）,low dose herb group（ n = 10）,positive control group（ n = 10） and model group（ n = 10）. Rabbits were made atherosclerosis models by high-fat diet except for the sham operation group. The sham operation group and the model group were given 10 ml/（ kg·d） normal saline by gavage. The positive control group was given 30 mg/（ kg·d） atorvastatin calcium by gavage. The high and low dose herb groups were given Tiaozhi Fang Ⅰ 12. 10 and 3. 02 g/（ kg·d） by gavage. After 3 months,the levels of rabbits＇ serum total cholesterol（ TC）,triglyceride（ TG）,low density lipoprotein cholesterol（ LDL-C）,high density lipoprotein cholesterol（ HDL-C）,superoxide dismutase（ SOD）,malonaldehyde（ MDA）,serum tumor necrosis factorα（ TNF-α）,interleukin 6（ IL-6）,intercellular adhesion molecule 1（ ICAM-1） and vascular cell adhesion molecule 1（ VCAM-1） in each group were determined. The expressions of aorta abdominalis low density lipoprotein cholesterol receptor（ LDL-R） and VCAM-1 mRNA were detected by reverse transcription-polymerase chain reaction（ RT-PCR） method. Results Compared with the model group,TC,TG,LDL-C and MDA in the high and low dose herb groups decreased; SOD level increased. The levels of TNF-α,IL-6,ICAM-1 and VCAM-1 and VCAM-1 mRNA expression in the high dose herb group decreased; LDLR mRNA expression increased（ P〈0. 05 or P〈0. 01）. Compared with the positive control group,SOD level in the high dose herb group increased; TNF-α level decreased（ P〉0. 05）. The difference of indexes between high and low dose herb groups was not significant（ P〈0. 05）. Conclusion Tiaozhi Fang Ⅰ might improve blood lipid,reduce serum inflammatory factor,up-regulate LDL-R mRNA and down-regulate VCAM-1 mRNA,which might be one o

关 键 词：动脉粥样硬化 调脂Ⅰ号方 炎症因子 血管细胞黏附分子 血脂 

分 类 号：R285.5[医药卫生—中药学]