千金子制霜前后提取物对大鼠肠道菌群的影响  

Effect of Semen Euphorbiae and Semen Euphorbiae Pulveratun extracts on intestinal flora in rats

作　　者：孙振阳 王英姿[1] 聂瑞杰 骆声秀 李文华[1] 张瑜[1] 张景珍 王思雨 

机构地区：[1]北京中医药大学,北京100102

出　　处：《中草药》2017年第21期4493-4496,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81673597)

摘　　要：目的研究千金子制霜前后提取物对正常大鼠肠道菌群的影响,为进一步阐明千金子制霜减毒机制提供参考。方法大鼠ig高、中、低剂量的千金子生品和霜品提取物,采用平板菌落计数法考察千金子制霜前后大鼠粪便中4类代表性肠道菌群双歧杆菌、乳酸杆菌、大肠杆菌、肠球菌的数量变化。结果平板菌落计数实验结果表明,ig千金子生品与霜品提取物后大鼠出现菌群失调现象,且千金子霜品较生品引起的菌群紊乱程度低,在低剂量给药时可使条件致病菌肠球菌和大肠杆菌的数量减少。结论千金子制霜前后提取物均会影响肠道菌群的生物多样性,影响肠道菌群平衡,且千金子制霜后对4类肠道菌群的作用减弱,引起肠道菌群紊乱程度减小,这与千金子霜品泻下作用缓和的研究结果相一致,表明从肠道微生态角度考察千金子制霜前后对肠道菌群的干预作用,可揭示制霜与肠道菌群数量变化可能存在相关性。Objective To study the effect of Semen Euphorbiae and Semen Euphorbiae Pulveratun on the microflora in rats gastrointestinal from the aspect of the intestinal microecology,so as to provide reference for further revealing mechanism of the attenuation after processing of Semen Euphorbiae.Methods Rats were fed with high,medium and low dose of Semen Euphorbiaeand Semen Euphorbiae Pulveratun,and the plate-counter method was used to examine the four types of intestinal flora in the feces of rats which are Bifidobacterium,Lactobacillus,Escherichia coli,and Enterococcus.Results After intragastric administration,the rat had the alteration of intestinal flora,and the degree of alteration of intestinal flora in the Semen Euphorbiae Pulveratun group was lower than the group of Semen Euphorbiae.In the low doses of group,the number of Enterococci and Escherichia coli was decreased.Conclusion Semen Euphorbiae and Semen Euphorbiae Pulveratun could affect the intestinal microbial diversity and the balance of intestinal flora.The effect of Semen Euphorbiae Pulveratun on four kinds of intestinal flora was weakened,and the degree of intestinal flora disturbance was reduced,which was consistent with the results of alleviation of the effect of Semen Euphorbiae Pulveratun.It was suggested that the intervention effect of Semen Euphorbiae on intestinal microflora could be clarified from the perspective of intestinal microecology,which could reveal possible correlation between Semen Euphorbiae Pulveratun and the changes of intestinal flora.

关 键 词：千金子 千金子霜 制霜减毒 双歧杆菌 乳酸杆菌 大肠杆菌 肠球菌 肠道菌群 

分 类 号：R285.5[医药卫生—中药学]