β-TCP／PLGA复合BMSCs联合高压氧修复海水浸泡兔桡骨骨缺损的作用研究  

Study on the effect ofβ-TCP/PLGA scaffolds seeded BMSCs combing with hyperbaric Oxygen on bone defect with seawater immersing in rabbit radius

作　　者：张淦 程迅生[1] 陈肖松[1] 马武秀 陈聪聪 符来想 

机构地区：[1]中国人民解放军第105医院骨科,合肥230031

出　　处：《重庆医学》2017年第34期4766-4770,共5页Chongqing medicine

基　　金：南京军区医学科技创新重点课题(11Z011)

摘　　要：目的探讨β-磷酸三钙/聚乳酸-羟基乙酸共聚物(β-TCP/PLGA)复合骨髓间充质干细胞(BMSCs)联合高压氧修复海水浸泡骨缺损的疗效。方法复苏和传代的BMSCs接种于β-TCP/PLGA支架上,构建组织工程骨。60只新西兰兔在双侧桡骨制作1.5cm的骨缺损,经海水浸泡3h后,分成4组,A组:空白对照组;B组:单纯BMSCs组;C组:BMSCs+高压氧组;D组:β-TCP/PLGA+BMSCs高压氧组。分别于术后4、8、12周分别X射线摄片处死后取桡骨。通过X线片、苏木素-伊红(HE)染色和免疫组织化学检测,评价各组海水浸泡骨缺损修复效果。结果影像学检查,术后12周A组骨缺损未完成修复,断端硬化;B组骨缺损部分修复;C组骨缺损基本完成修复,髓腔未通;D组骨缺损完成修复,髓腔再通。各个时间点骨痂灰度值D组>C组>B组>A组(P<0.05)。组织学观察,术后12周,A组少量板层骨;B组少量板层骨形成,少量骨小梁生成;C组大量板层骨形成,骨小梁排列欠规则;D组大量板层骨形成,骨小梁排列规则。免疫组织化学检测各组表达骨钙素(OCN)强度,术后4周最强,术后8周减弱,术后12周达到最低。术后4周和8周,OCN表达强度D组>C组>B组>A组(P<0.05),术后12周各组差异无统计学意义(P>0.05)。结论β-TCP/PLGA复合BMSCs联合高压氧是修复海水浸泡骨缺损的有效方法之一。Objective To investigate therapeutic effects of β-TCP/PLGA scaffolds seeded bone marrow mesenchymal stem cells （BMSCs） combing with hyperbaric oxygen （HBO） on bone defect with seawater immersing in rabbit radius. Methods BMSCs were seeded into the β-TCP/PLGA scaffolds to construct tissue engineering bone. 60 New Zealand rabbits were created 1.5 cm bone defect in bilateral radius, and then bilateral wound limbs were immersed in seawater for 3 h. After debridement, all rabbits were randomly divided into 4 groups. In group A nothing was implanted into the bone defect. Only BMSCS was implanted in group B. BMSCs ＋ HBO was given in group C. β-TCP/PLGA BMSCs＋ HBO was given in group D. At postoperative 4,8,12 weeks rabbits were sacrificed after radiography radius. X-ray radiography, HE staining, immunohistochemical observation were used to evaluate repai- ring effect of bone defect with seawater immersing. Results Radiographic analysis demonstrated that the bone defects were completely repaired with recanalization of the medullary cavity in group D, bone defects were partially repaired with partial recanalization of the medullary cavity in group C, bone defects were incompletely repaired in group B; bone defect repair unfinished and the broken ends were sclerous in group A. Each time point group D〉group C〉group B〉group A in callus grey value （P〈0. 05）. HE staining indicated after 12 weeks,a small amount of lamellar bone formated in group A;a small amount of lamellar bone formated in group β; a large amount of lamellar bone formated in group C;a large amount of lamellar bone formated in group D. Immunohistoeheroical result indicated at postoperative 4 weeks, the expressions of osteocalcin（OCN） in each group were at high levels, the expressions decreased significantly at postoperative 8 weeks,the expressions were at a low level at postoperative 12 weeks. At postoperative 4 and 8 weeks,the expression levels of OCN were group D〉group C〉group B〉group A （P〈0. 05） ,there was no signif

关 键 词：β-磷酸三钙/聚乳酸-聚羟乙酸 骨缺损 海水浸泡 高压氧 骨髓间充质干细胞 

分 类 号：R687.3[医药卫生—骨科学]