玫瑰花挥发油对小鼠急性心肌缺血损伤的保护作用研究  

Protective effects of essential oil of Uygur medicine rose on acute myocardial ischemia injury in mice

作　　者：宋旺弟 袁长胜[1] 崔婷婷[1] 申静 陈文[1] 

机构地区：[1]石河子大学药学院,新疆石河子832000

出　　处：《中草药》2017年第22期4701-4706,共6页Chinese Traditional and Herbal Drugs

基　　金：新疆兵团英才计划

摘　　要：目的探讨玫瑰花挥发油抗小鼠急性心肌缺血损伤的心肌保护作用及机制。方法采用ip异丙肾上腺素的方法制备小鼠急性心肌缺血模型,以玫瑰花挥发油(160、80、40 mg/kg)进行药物干预,通过HE染色和TTC染色观察小鼠心肌组织病理变化和心肌损伤程度,观察药物对小鼠心肌组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)以及小鼠血清中肌酸激酶同工酶(CK-MB)和乳酸脱氢酶(LDH)活性的影响。结果 HE染色结果表明,玫瑰花挥发油160、80、40 mg/kg均可以明显改善小鼠心肌组织的病理性损伤情况;TTC染色结果发现,玫瑰花挥发油160、80、40 mg/kg可以显著减小小鼠心肌缺血区的面积;与模型组相比,玫瑰花挥发油160、80 mg/kg组小鼠心肌组织中SOD、GSH-Px的酶活性显著增强(P<0.05、0.01),血清中LDH和CK-MB水平显著降低(P<0.05、0.01)。结论玫瑰花挥发油对异丙肾上腺素所致的小鼠急性心肌缺血有一定的保护作用,作用机制可能与玫瑰花挥发油抑制氧自由基的产生、增强抗氧化酶活性,以及降低脂质过氧化损伤有关。Objective To study the protective effect of rose（Rosae Rugosae Flos） essential oil on acute myocardial infarction in mice, and explore its mechanism. Methods The model of acute myocardial ischemia in mice was established by intraperitoneal injection of isopropyl adrenaline. The myocardial tissue pathological changes and the degree of myocardial injury in mice were observed by HE staining and TTC staining. Activities of super oxide dismutase（SOD） and glutathione peroxidase（GSH-Px） in myocardial tissue of mice were determined, and activities of creatine kinase isoenzyme（CK-MB） and lactate dehydrogenase（LDH） in serum of mice were determined as well. Results HE staining results showed that rose essential oil group（160, 80, and 40 mg/kg） can significantly improve myocardial tissue pathological injury in mice; TTC staining results showed rose essential oil（160, 80, and 40 mg/kg） can significantly reduce the myocardial ischemia areas in mice. Compared with model group, SOD and GSH-Px in the myocardial tissue of mice significantly increased（P〈0.05, 0.01）, while enzyme activity of serumLDH and CK-MB significantly reduced in rose essential oil group（P〈0.05, 0.01）. Conclusion Rose essential oil has certain protective effect on acute myocardial infarction injury and its mechanism may be associated with inhibiting oxidative damage, enhancing activities of anti-oxidant enzymes, and reducing the lipid peroxidation damage.

关 键 词：玫瑰花 挥发油 急性心肌缺血 超氧化物歧化酶 谷胱甘肽过氧化物酶 肌酸激酶同工酶 乳酸脱氢酶 

分 类 号：R285.5[医药卫生—中药学]