Expression and Response Analysis of NCED Gene of Zoysia japonica in Initial Infection Stage of Rhizoctonia solani  

作　　者：Li Linkun He Xusheng Zeng Huiming 

机构地区：[1]Turfgrass Research Institute, Beijing Forestry University, Beijing 100083, China

出　　处：《Plant Diseases and Pests》2017年第5期23-26,共4页植物病虫害研究（英文版）

基　　金：Supported by National High Technology Research and Development Program("863"Program) of China(2013AA102607)

摘　　要：[Objective] The paper was to analyze the expression and response mechanism of NCED gene of Zoysia japonica in initial infection stage of Rhizoctonia solani. [Method] The NCED gene fragments of Z. japonica‘Zenith＇were screened and cloned on the basis of transcriptome data. The expression patterns of NCED gene in initial infection stage of R. solani（ 0-48 h） were obtained via real-time quantitative PCR. [Result] The expression level of NCED gene in stress treatment was higher than that without infection,which first increased then decreased with the extension of time,and reached the maximum at 36 h. Further detection of ABA level in‘Zenith＇roots showed that the change trend of ABA level within 0-48 h was consistent with the expression pattern of NCED gene,and flattened out after 48 h. [Conclusion] NCED gene regulates the synthesis of phytohormone ABA and probably further involves in plant disease resistance.[Objective] The paper was to analyze the expression and response mechanism of NCED gene of Zoysia japonica in initial infection stage of Rhizoctonia solani. [Method] The NCED gene fragments of Z. japonica‘Zenith＇were screened and cloned on the basis of transcriptome data. The expression patterns of NCED gene in initial infection stage of R. solani（ 0-48 h） were obtained via real-time quantitative PCR. [Result] The expression level of NCED gene in stress treatment was higher than that without infection,which first increased then decreased with the extension of time,and reached the maximum at 36 h. Further detection of ABA level in‘Zenith＇roots showed that the change trend of ABA level within 0-48 h was consistent with the expression pattern of NCED gene,and flattened out after 48 h. [Conclusion] NCED gene regulates the synthesis of phytohormone ABA and probably further involves in plant disease resistance.

关 键 词：Zoysia japonica Steud Rhizoctonia solani NCED gene Quantitative expression 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]