赛鸽新城疫病毒南京分离株的生物学特性及其F和HN基因的分析  

Biologic characterization and F and HN gene analysis of racing pigeon Newcastle disease virus strain from Nanjing

作　　者：熊晓妍 孙兴臣 李敏婕[1] 耿文学[1] 万玉萌 孔志龙 陈闻[1] 许家荣[1,2] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]南京千里光生物技术有限公司,江苏南京210095

出　　处：《中国兽医科学》2017年第11期1363-1370,共8页Chinese Veterinary Science

基　　金：句容市科委项目(NY2013029);镇江市农业科技支撑项目(SBK2014022021);南京农业大学教改项目(2013Y021);南京农业大学动物医学院教育教学改革研究项目;赛鸽疾病诊断的研究与防治项目

摘　　要：从南京市某赛鸽公棚发病鸽脑组织中分离到1株鸽新城疫病毒,命名为Pi/NJ/CH/4416/2016(简称ND4416)。各项生物学特性试验结果表明,该分离株为鸽新城疫强毒,对鸽具有高致病性,对鸡的致病性相对较低。经RT-PCR扩增、测序得到分离株的F基因和HN基因序列。F基因分析表明,其裂解位点的氨基酸序列为^(112)R-R-Q-K-R-F117,是典型的强毒株共有序列。F、HN基因遗传进化分析发现,本次分离株的基因型为Class II,VIb亚型,且与目前国内流行株属于同一遗传分支,这些毒株与比利时流行株PPMV-1/Belgium/11-09620/2011(JX901124.1)的亲缘关系相近,推测近年国内流行毒株可能来源于比利时。本次分离毒株与弱毒疫苗株La Sota(AF077761.1)的遗传距离较远。生物信息学分析显示,该鸽源分离株HN蛋白第491~500位的一个线性抗原表位发生了改变,血清学试验也显示本毒株与La Sota疫苗株的抗原性差异明显,在一定程度上说明该鸽源毒株已经发生了抗原性变异。One strain of pigeon Newcastle disease virus（NDV） was isolated from a diseased racing pigeon loft suspected to be infected with NDV in Nanjing. This NDV isolate was named Pi/NJ/CH/4416/2016（ND4416） and purified by using specific pathogen-free（SPF） eggs.Biological tests showed that this isolate showed high pathogenicity to pigeons,but lower to chickens.The resulting RNA samples were converted to c DNAs, followed by PCR amplification and sequencing of F and HN genes.The cleavage site of the fusion protein（F） was112 RRQKRF117,a feature generally associated with virulent NDV strains.Phylogenetic analysis based on F and HN gene sequences showed that this strain belonged to Class Ⅱ,Ⅵb subgenotype.In addition,Pi/NJ/CH/4416/2016 and other strains prevailing in China were more closely related to the PPMV-1/Belgium/11-09620/2011（JX901124.1） strain. It supported that epidemic pigeon NDV isolates in China might originate from Belgium. Pi/NJ/CH/4416/2016 had a far genetic relationship from the classic vaccine strain La Sota（AF077761.1）.Bioinformatics analysis revealed that a linear epitopeconsisting of amino acids 491-500 of HN protein of this pigeon strain was different from La Sota.In addition,hemagglutination inhibition test revealed a noticeable antigenic difference between them.

关 键 词：赛鸽 鸽新城疫 生物学特性 基因型Ⅵ亚型 F基因 HN基因 

分 类 号：S858.393[农业科学—临床兽医学]