牛源非脱羧勒克菌的分离鉴定及其耐药基因的检测  

Isolation and identification of Leclercia adecarboxylata from cattle and detection of resistance gene

作　　者：田佳琪[1] 董文龙[1] 王巍[1] 王羽[1] 盛宇轩 张喜庆[1] 稽少泽 马红霞[1] 高云航[1] 

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118

出　　处：《中国兽医科学》2017年第11期1399-1405,共7页Chinese Veterinary Science

基　　金：吉林省现代农业产业技术体系建设专项(2015-24);吉林省动物疾病防治研究创新团队项目(20111820)

摘　　要：为诊断导致乳牛死亡的病原菌,于病死牛腹腔积液中分离得到1株具有致病性的优势菌株。经16S r DNA检测鉴定为非脱羧勒克菌。动物回归试验中,病牛肺、心外膜、喉头病理变化明显。以15种抗生素进行药敏试验,该非脱羧勒克菌对大环内酯和氨基糖苷类药物均呈显著耐药。耐药基因检测中大环内酯类耐药基因与氨基糖苷耐药基因均呈显性表达。In order to diagnose the pathogenic bacteria of death cattle,a dominant pathogenic strain was isolated from ascites of a dead cattle. The strain was identified as Leclercia adecarboxylata by 16 S r DNA gene. The lung,epicardium,larynx of death cattle had obvious lesions in regression animal experiment.In the drug sensitivity test,macrolide and aminoside were significantly resistant to15 antibiotics.And the macrolide resistance genes and aminoside resistance genes of L.adecarboxylata were positive.

关 键 词：非脱羧勒克菌 分离鉴定 最小抑菌浓度 大环内酯、氨基糖苷耐药基因 

分 类 号：S852.612[农业科学—基础兽医学]