绵羊下丘脑催产素神经元分布的研究  

Study on distribution of oxytocin expression in ovine hypothalamus

作　　者：郭志凯[1] 范奎奎[2] 潘登[2] 李婷[2] 李强[2] 钱英红 杜晨光[1,2] 

机构地区：[1]内蒙古农业大学职业技术学院,内蒙古土默特右旗014109 [2]内蒙古农业大学兽医学院,内蒙古呼和浩特010018 [3]内蒙古自治区农牧业科学院,内蒙古呼和浩特010031

出　　处：《中国兽医科学》2017年第11期1457-1460,共4页Chinese Veterinary Science

基　　金：国家自然科学基金项目(31160455;31660701)

摘　　要：催产素(oxytocin,OXT)在动物分娩、维持哺乳方面具有重要作用,近年来发现OXT对能量代谢亦具有积极作用。尽管OXT在小鼠、大鼠、猕猴等动物脑部有广泛的分布表达,但OXT在绵羊下丘脑的表达部位和神经投射通路尚无报道,成为限制研究其生物学功能的瓶颈之一。本试验以绵羊下丘脑为主要研究材料,运用免疫荧光技术明确了OXT神经元在下丘脑的表达分布。结果显示,OXT神经元集中分布在绵羊下丘脑室旁核(paraventricular nucleus,PVN)区域,在视上核(supraoptic nucleus,SON)有零星表达,且均具有较为明晰神经元胞体及纤维结构,这种分布与小鼠的表达部位呈现高度一致。上述结果为进一步研究OXT在绵羊能量代谢方面发挥的生物学作用奠定了免疫学基础。Oxytocin（OXT）,discovered in recent years,plays an important role in maintaining animal birth and lactation.It also has a positive effect on the energy metabolism.Although OXT is widely distributed in the brains of mice,rats,monkeys and other animals,it has not been reported on expression site of ovine hypothalamus and neural projection pathway,which has become a bottleneck restricting of studies on its functions.In this experiment,the ovine hypothalamus was used as the main materials and the expression and distribution of OXT neurons in hypothalamus was detected by using immunofluorescence technique.The results showed that the distribution of OXT neurons in ovine hypothalamic paraventricular nucleus（PVN）and supraoptic nucleus（SON）was clear and the OXT neurons had fiber structure,and the distribution and expression site in mice showed a high degree of consistency.The above-mentioned results laid the immunological basis for the further studies of OXT＇s biological fuctions in ovine energy metabolism.

关 键 词：催产素 绵羊 下丘脑 室旁核 神经元 

分 类 号：S852.13[农业科学—基础兽医学]