转化生长因子-β1对大鼠同种胰岛移植物的影响  

The effect of transforming growth factor-β1 on rat islet allograft

作　　者：范骥[1] 王春友[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院胰腺外科中心,武汉430022

出　　处：《中华实验外科杂志》2002年第5期423-425,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目(30170911)

摘　　要：目的　研究转化生长因子 β1 (TGF β1 )对大鼠同种胰岛移植物体内外的内分泌功能及存活的影响。方法　构建TGF β1的真核表达载体 pcDNA3 TGF ,并将其转染纯化后的Wistar大鼠胰岛 ,逆转录 聚合酶链式反应 (RT PCR)检测TGF β1和大鼠胰岛素 (Ins1 )在实验组和对照组胰岛中的表达 ,放射免疫法 (RIA)检测体外培养液中的胰岛素含量 ;链脲霉素 (STZ) 60mg/kg体重腹腔内注射制作SD大鼠模型 ,分别将实验组和空白对照组Wistar胰岛移植到受体SD大鼠的肾包囊下 ,观察其在体内逆转糖尿病的作用以及作用时间的长短 ,并检测受体大鼠的糖耐量。结果　实验组胰岛有TGF β1和Ins1的表达 ,而对照组仅有Ins1的表达 ;实验组体外培养液中的胰岛素含量在基础相和刺激相分别为 (3 .86± 1 .2 4 ) μg/L和 (8.43± 1 .59) μg/L ,对照组体外培养液中的胰岛素含量在基础相和刺激相分别为 (4.1 3± 1 .45) μg/L和 (8.95± 1 .74) μg/L ,组间差异无显著性 (P>0 .0 5) ,而组内差异有显著性 (P <0 .0 5) ;实验组平均正常血糖维持时间 (2 6 .8± 2 .9)d比对照组的 (1 3 .2± 4 .5)d显著延长。对照组糖耐量曲线均明显高于实验组。结论　TGF β1对胰岛移植物的内分泌功能无损害作用 。Objective To investigate the effect of transforming growth factor β1 on secretion function and survival of rat islet allograft in vivo and in vitro.Methods Expressing vector of rat TGF β1 was constructed and transfected to purified Wistar rat islets. Expression of TGF β1 and rat insulin1 gene (Ins1) in experimental and control groups was detected by RT PCR; The SD rat diabetic model was developed by intra abdominal injection of streptozotocin 60?mg/kg. Insulin of cultured islets at fundamental and stimulated phase in vitro was detected by RIA; Islet allotransplantation was performed under recipient SD rats'renal subcapsule. The level of blood glucose was recorded before and after transplantation. The glucose tolerance was also tested.Results Expression of both TGF β1 and Ins1 was detected in the experimental group, but only Ins1 was detected in the control one; The secretion amount of insulin in the experiment and control islets was (3.86±1.24)?μg/L and (4.13±1.45)?μg/L at fundamental phase, (8.43±1.59)?μg/L and (8.95±1.74)?μg/L at stimulated phase, respectively. There was no significant difference between the two groups, but there was intra group significant difference. The mean normglymia time in the experimental group was (26.8±2.9) days, significantly longer than in the control group (13.2±4.5) days. The glucose tolerance curve in the control group was significantly higher than in the experimental group.Conclusion TGF β1 can prolong the survival of islet allograft while it does not impair graft's endocrine function.

关 键 词：转化生长因子-Β1 大鼠 同种胰岛移植物 影响 胰岛移植 

分 类 号：R657.5[医药卫生—外科学]