茜草素介导BK_(Ca)通道beta亚基功能舒张肾叶间动脉降低SHR收缩压  

Alizarin induced the function of beta subunits of BK_(Ca) channel relaxed interlobar renal artery and reduced SHR systolic pressure

作　　者：章艳萍 王元媛 干艳捷 王家宁[2] 张传林 李晓雯 

机构地区：[1]湖北医药学院附属人民医院心脏中心,湖北省十堰市442000 [2]湖北医药学院临床医学研究所,湖北省十堰市442000 [3]湖北医药学院附属人民医院内分泌科,湖北省十堰市442000

出　　处：《实用医学杂志》2017年第22期3706-3709,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:81270221);湖北省十堰市科技局资助项目(编号:15Y34;16K72)

摘　　要：目的研究茜草素对自发性高血压大鼠(SHR)肾叶间动脉平滑肌细胞BK_(Ca)通道功能和表达的变化。方法应用尾动脉测压仪、压力机动图技术、膜片钳技术和Western Blot技术分别观察茜草素干预前后SHR收缩压,肾叶间动脉血管直径,BK_(Ca)通道电流和beta亚基表达的变化。结果 (1)茜草素干预后,SHR收缩压较干预前降低(P<0.01);(2)ibTX抑制茜草素对SHR肾叶间动脉血管段的舒张作用(P<0.01);(3)茜草素增强SHR肾叶间动脉平滑肌细胞BK_(Ca)通道介导的电流(P<0.05);(4)茜草素干预第21天,BK_(Ca)通道beta亚基表达上调(P<0.01)。结论茜草素通过调节BK_(Ca)通道beta亚基功能,引起平滑肌细胞超极化,肾叶间动脉舒张和SHR收缩压降低。Objective To investigate the effect of alizarin on BKCa channel beta subunit and electrophysiological characteristics in interlobar renal artery of SHR. Methods Tail artery pressure measurement instrument, pressure myograph system, whole cell patch clamp technique and Western blot were used to measure the change of systolic pressure of SHR, renal interlobar arterial diameter, single vascular smooth muscle cell electrophysiological characteristics and expression of the BKCa channel beta subunit before and after alizarin intervention, respectively. Results （ 1 ） SHR systolic pressure was decreased after alizarin intervention （P 〈 0.01 ）. （2） ibTX inhibited aliza- rin-mediated renal interlobar arterial relaxation （P 〈 0.01 ）. （3） Alizarin enhanced BKCa channel-mediated out- ward currents （P 〈 0.05）. （4） Alizarin up-regulated BKCa channel beta subunits after alizarin intervention （P 〈 0.01 ）. Conclusion Alizarin reduced SHR systolic pressure and relaxed interlobar renal artery by enhancement BKCa currents via mediation of the function of beta subunits.

关 键 词：茜草素 肾叶间动脉 BKCA通道 平滑肌细胞 全细胞膜片钳 

分 类 号：R285.5[医药卫生—中药学]