原位预处理-薄层扫描法同时测定唇香草中熊果酸和齐墩果酸的含量  

Simutaneous determination of ursolic acid and oleanolic acid in Ziziphora clinopodioides Lam. by in situ pretreatment-thin layer chromatography scanning

作　　者：丁文欢 杨添尹 张雪佳[3] 徐海燕[4] 周晓英 

机构地区：[1]新疆医科大学中心实验室,乌鲁木齐830054 [2]新疆医科大学第五附属医院检验科,乌鲁木齐830054 [3]中国药科大学中药学院,南京211198 [4]新疆医科大学中医学院,乌鲁木齐830054 [5]新疆医科大学药学院化学教研室,乌鲁木齐830054

出　　处：《国际药学研究杂志》2017年第8期812-816,共5页Journal of International Pharmaceutical Research

基　　金：新疆维吾尔自治区科技计划项目(201233134)

摘　　要：目的建立维药唇香草中熊果酸(UA)和齐墩果酸(OA)的含量测定方法,并测定不同产地、不同采收期以及不同部位的唇香草中UA和OA的含量。方法采用双波长扫描法,对点于硅胶G板的样品进行原位预处理,以环己烷-氯仿-乙酸乙酯-甲酸(20∶5∶8∶0.1)为展开剂,测定波长为530 nm,参比波长为700 nm。结果该法中的UA和OA分离效果较好,可被同时检识。采自新疆18个不同产地的唇香草中UA和OA的平均含量分别为(1.84±0.41)和(2.82±0.89)mg/g;在相同年份5月初至月末的采收期UA和OA含量呈现春末到夏初期上升,其后下降;唇香草不同部位中UA和OA的含量为叶中最高,花次之,茎最低。结论方法简便、快速、准确,结果可为唇香草的优质资源评价提供基础数据。Objective To establish a method for the simultaneous determination of ursolic acid（UA）and oleanolic acid（OA）in Ziziphora clinopodioides Lam.,and the quantitative determination the UA and OA contents in the different Z. clinopodioides plant samples collected with various parts of the plant at different times,from different regions of Xinjiang,China. Methods Dual wavelength scanning method was used for the quantification of UA and OA spots on a silica gel G plate in the TLC analysis. The samples loaded on the silica gel G plate were in situ treated with the 1% iodine solution in dichloromethane,and then the plate was developed using cyclohexane,cyclohexane-chloroform-ethyl acetate-formic acid（20∶5∶8∶0.1）as the developing solvent. In the dual wavelength scanning,the measurement wavelength was 530 nm and the reference wavelength was 700 nm. Results The UA and OA spots in samples were well separated on the TLC plate and could be simultaneously quantified by the present method. The average contents of UA and OA in Z. clinopodioides plant samples from 18 different areas were（1.84 ± 0.41）and（2.82 ± 0.89）mg/g,respectively. The contents of UA and OA in the plant increased from late spring to early summer and then decreased thereafter. As to the different parts of the plant,the contents of UA and OA were highest in leaves and lowest in stems. Conclusion The method is simple,fast and accurate. The present results provided basic data for further evaluation of the quality of Z. clinopodioides resources.

关 键 词：唇香草 薄层扫描 熊果酸 齐墩果酸 

分 类 号：R284.1[医药卫生—中药学]