当归挥发油对自发性高血压大鼠ACE2基因表达的影响  

The effects of Angelica on ACE2 gene in spontaneously hypertensive rats

作　　者：伊琳[1] 曲强[2] 纪禄风 石向慧 王立红[1] 顾远晖[2] 

机构地区：[1]甘肃中医药大学,甘肃兰州730000 [2]甘肃省人民医院

出　　处：《中国老年学杂志》2017年第22期5507-5509,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金(No.81460669);甘肃省中医药管理局项目批准号(GZK-2016-40);甘肃省自然科学基金(No.1310RJZA084)

摘　　要：目的探讨当归挥发油对自发性高血压大鼠(SHR)心肌血肌紧张素转换酶(ACE)2基因表达的影响及作用机制。方法将40只8周龄雄性SHR随机分为模型组,当归挥发油组(低、高)和卡托普利组,并以同周龄的Wistar大鼠作为正常对照组。分别灌胃4 w后,取大鼠心肌组织,HE染色观察心肌组织形态学的变化实时荧光定量PCR法检测ACE2 mRNA表达量,Western印迹检测ACE2蛋白的表达水平。结果当归可降低SHR血压;HE染色显示,与模型组相比较,当归挥发油各组病变分别有不同程度的减轻;与模型组相比,当归高、低剂量组ACE2 mRNA及蛋白表达水平均明显升高(P<0.01)。结论当归挥发油对SHR血压有调节作用,可能通过ACE2基因发挥降压作用。Objective To study the influence of Angelica sinensis（ oliv.） Diels on ACE2 gene in spontaneously hypertensive rats（ SHR）.Methods All the rats were divided into Angelica,model,captopril groups,weekly-old Wistar rats as normal control group,The systolic blood pressure（ SBP） of the tail in different groups before and after treatment were measured.After 4 weeks,cardiac tissue of the rats were taken out,HE stain was used to observe the pathological changes of cardiac tissue,the mRNA level of ACE2 was detected by RT-PCR,the protein expression of ACE2 was tested by Western blot. Results Angelica reduced SBP in SHRs. Myocardial fibers of Angelica group were thinned and organized with sharply defined stripes structure. The ACE2 mRNA and protein expressions were significant increased in Angelica group（ P〈0. 01）.Conclusions The essential oil of Angelica sinensis could reduce the blood pressure through up-regulating the expression of ACE2.

关 键 词：当归挥发油 高血压 血管紧张素转换酶(ACE)2 

分 类 号：R2-031[医药卫生—中西医结合]