银杏叶提取物对坐骨神经损伤后感觉神经元超微结构的影响  

Influence of ginkgo biloba extract on ultrastructural changes of sensery neurons following sciatic nerve injury in rats

作　　者：薛锋[1] 顾玉东[1] 李继峰 陈德松 钟慈声[2] 

机构地区：[1]复旦大学附属华山医院手外科卫生部手功能重建重点实验室,上海200040 [2]复旦大学医学院生物物理学教研室

出　　处：《中华实验外科杂志》2002年第5期452-453,共2页Chinese Journal of Experimental Surgery

基　　金：国家"973"基础研究项目(G19990 54 2 0 2 );上海市科学技术发展基金资助项目 ( 10DJ90 14 )

摘　　要：目的　研究银杏叶提取物对大鼠坐骨神经损伤后相应脊神经节感觉神经元超微结构的影响。方法　将SD大鼠 36只随机分为银杏叶提取物组和生理盐水组。制作左侧坐骨神经部分切除及反折结扎模型。术后两组分别经腹腔注射银杏叶提取物 (1 0 0mg·kg 1 ·d 1 )和同体积的生理盐水。 2、4、6周取材 ,电镜观察L4 及L5脊神经节感觉神经元超微结构形态变化 ,体视学方法计算并比较两组感觉神经元线粒体、内质网及溶酶体体积密度变化。取L6 神经节 ,光镜下计数并计算神经节感觉神经元存活率。结果　实验组超微结构形态变化明显轻于对照组。术后 2、4、6周神经元线粒体、内质网及溶酶体体积密度值均小于对照组 ,其差异有显著性 (P <0 .0 5)。实验组 2、4及 6周感觉神经元存活率为 85 .58%、82 .45 %和 79.1 1 % ,存活率大于对照组 (P <0 .0 5)。结论　银杏叶提取物 。Objective To investigate the protective effect of Ginkgo biloba extract (EGb761) on sensory neurons of dorsal root ganglion after peripheral nerve injury.Methods Thirty eight SD rats were subjected to left sciatic nerve partal resection and both ends ligation. Two groups were divided. One for EGb761 peritoneal injection (100?mg·kg -1 ·d -1 ) and another for 0.9% saline of the same volume. At 2, 4 and 6 weeks postoperatively, L4,5 dorsal ganglias (left) were harvested and ultrastractural changes of sensery neurons were observed. Morphological analysis and volume density calculations of rough endoplasmic reticulum, mitochondrion and lysosome were made. The L6 dorsal root ganglia were removed bilaterally and were cut in 5?μm section which stained with cresyl violet. Neurons were counted and survival rate was calculated.Results Ultrastructural changes in experimental groups were milder. Two, 4, 6 weeks after operation, the volume density values of mitochondrion, endoplasmic reticulum and lysosome in the neurons were all less than in the control group (P<0.05). In the experimental group, the survival rate of sensory neurons at 2, 4 and 6 weeks was 85.58%, 82.45% and 79.11%, respectively, significantly higher than in the control group (P<0.05).Conclusion EGb761 might have certain protective effects on sensery neurons after peripheral nerve injury.

关 键 词：周围神经 感觉神经元 银杏 中药 药理 

分 类 号：R285.5[医药卫生—中药学]