HIV感染者外周血滤泡辅助性T细胞亚群变化特征研究  

Changes of peripheral follicular helper T cell subsets in HIV infected patients

作　　者：赵爽[1] 陈威巍[2] 赵敏[2] 

机构地区：[1]北京大学302临床医学院,100039 [2]解放军第302医院感染性疾病诊疗与研究中心,北京100039

出　　处：《中华实验和临床病毒学杂志》2017年第5期392-396,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金（81470097,81471577）

摘　　要：目的 探讨HIV感染者外周血滤泡辅助性T细胞（Tfh）及其亚群分布情况以及与病情进展的相关性.方法 采用流式细胞术检测20例未经治疗的HIV感染者,20例经HAART后病毒完全抑制的HIV感染者,以及15例健康人外周血Tfh及其亚群的频率,分析其与病毒载量、CD4 T淋巴细胞计数及血浆丙种免疫球蛋白浓度的相关性.结果 与健康对照组相比,未治疗组外周血Tfh17频率明显降低（P＜0.05）,与血浆丙种免疫球蛋白浓度呈负相关,并与外周血CD4 ＋T细胞计数呈正相关,与病毒载量呈负相关;Tfh2频率与血浆丙种免疫球蛋白及病毒载量呈正相关,并与外周血CD4＋T细胞计数呈负相关;Tfh1频率在HIV感染者中明显升高（P＜0.05）,与在未治疗组中与血浆丙种免疫球蛋白浓度和病毒载量呈负相关,与外周血CD4＋T细胞计数呈正相关.结论 Tfh亚群分布失衡,可能与HIV感染者持续性免疫激活状态和疾病进展有关.Objective To investigate the changes of the frequencies of follicular helper T （Tfh） cell subsets in peripheral blood of HIV-1 infected patients and its relevance to the disease severity.Methods Twenty untreated HIV ＋ patients,20 HAART-suppressed patients and 15 health controllers are enrolled in this study.The frequencies of Tfh and its subsets were examined by flow cytometry.Results The frequency of Tfh17 subset in peripheral blood decreased significantly in untreated HIV-1 ＋ patients as compared with those of healthy controls and HAART-suppressed patients （P 〈 0.05）.In addition,the frequency of this subset were positively correlated with CD4 ＋ T cell counts in both untreated and treated HIV ＋ patients,and were inversely correlated with viral load and plasma-immunoglobulin in untreated HIV ＋ patients.The frequency of Tfh2 subset,however,were inversely correlated with CD4 ＋ T cell counts and positively correlated with viral load and plasma-immunoglobulin in untreated HIV ＋ patients.The frequency of Tfh1 subset in peripheral blood increased significantly in untreated and treated HIV-1 ＋ patients as compared with those of healthy controls （P 〈 0.05） and were inversely correlated with viral load and plasmaimmunoglobulin in untreated HIV ＋ patients and positively correlated with CD4 ＋ T cell counts.Conclusions The abnormal distribution of Tfh subests may play a role in the progression of HIV infection and its characteristic immune activation.

关 键 词：HIV感染 滤泡辅助性T细胞 滤泡辅助性T细胞17 滤泡辅助性T细胞2 滤泡辅助 T细胞1 

分 类 号：R512.91[医药卫生—内科学]