不均一性核糖核蛋白A2／B1在脂多糖诱导的血管内皮细胞通透性增加中的作用  

Effect of heterogeneous ribonucleoprotein A2/B1 on lipopolysaccharide 4nduced permeability of vascular endothelial cells

作　　者：王雷 陈依 朱科明 

机构地区：[1]海军军医大学附属长海医院麻醉学部,上海200433

出　　处：《国际麻醉学与复苏杂志》2017年第11期1018-1022,1035,共6页International Journal of Anesthesiology and Resuscitation

摘　　要：目的探讨不均一性核糖核蛋白A2／B1（heterogeneous ribonucleoprotein A2／B1，hnRNPA2／B1）在脂多糖（lipopolysaccharide, LPS）刺激的人脐静脉内皮细胞（human umbilical vein endothelial cells, HUVECs ）通透性中的作用。方法以HUVECs为研究对象，筛选出具有hnRNPA2／B1基因沉默作用的小干扰RNA（small interfering RNA，siRNA），按不同处理方法实验分为转染阴性对照siRNA组（NCsiRNA组）、转染hnRNPA2／B1 siRNA组（hnRNPA2／B1 siRNA组）、转染阴性对照siRNA组并进行LPS干预组（NCsiRNA组-LPS组）、转染hnRNPA2／B1siRNA组并进行LPS干预组（hnRNPA2／B1 siRNA组。LPS组），分别给予或不给予LPS（1mg／L）刺激，检测不同时间点（0、6、12、24h）各组细胞跨膜电阻抗（transendothelial electrical resistance, TEER）值，检测LPS刺激12h后细胞表面血管内皮钙黏蛋白（vascular endothelial cadherin，VE-cadherin）及细胞间黏附分子-1（intercellular adhesion molecule．1，ICAM-1）蛋白表达变化。结果在相同剂量LPS诱导下，hnRNPA2／B1siRNA—LPS组HUVECs的TEER在12h低于NCsiRNA-LPS组（P〈0．05）；在LPS刺激12h后，hnRNPA2／B1siRNA-LPS组HUVECs表面VE-cadherin表达明显低于NCsiRNA-LPS组，ICAM-1表达明显高于NCsiRNA-LPS组（P〈0．05）。结论hnRNPA2／B1可能通过调节VE-eadherin及ICAM-1蛋白表达影响血管内皮细胞的通透性，发挥血管内皮屏障保护功能。Objective To investigate the effect of heterogeneous ribonucleoprotein A2/B1 （hnRNP A2/B1） on the human umbilical vein endothelial cells （HUVECs） permeability induced by lipopolysaccharide（LPS）. Methods HUVECs were used in this study, small interfering RNA （siRNA） with hnRNP A2/B1 gene silencing was screened. The samples were divided into four groups： NC siRNA group, hnRNP A2/B1 siRNA group, NC siRNA-LPS group and hnRNP A2/B1 siRNA-LPS group. The transendothelial electrical resistance （TEER） of HUVECs was detected at 0, 6, 12 h and 24 h. The expression of vascular endothelial cadherin （VE- cadherin） and intercellular adhesion molecule-1 （ICAM-1） were measured at 12 h. Results After LPS treatment, the TEER values of HUVECs in hnRNP A2/B1 siRNA-LPS group was significantly decreased compared with values of NC siRNA-LPS group at 12 h （P〈0.05）. The expression level of VE-cadherin in huRNP A2/B1 siRNA-LPS group was lower than the level in the NC siRNA-LPS group, and the expression level of ICAM-1 was increased in hnRNP A2/B1 siRNA-LPS group compared with the level of NC siRNA- LPS group（P〈0.05）. Conclusions hnRNP A2/B1 may play an important role in vascular endothelial permeability via regulating the expression of VE-cadherin and ICAM-1, thus protecting vascular endothelial barrier function.

关 键 词：不均一性核糖核蛋白A2/B1 脂多糖 内皮通透性 细胞表面血管内皮钙黏蛋白 

分 类 号：R459.7[医药卫生—急诊医学]