分子印迹基质固相分散-液相色谱法测定土壤中毒死蜱及代谢物残留量  

Determination of Chlorpyrifos and its Metabolite in Soil by Molecularly Imprinted Matrix Solid Phase Dispersion Combined with HPLC

作　　者：周梦春 于福新[2] 向茂英 周华伦 王沫[3] 

机构地区：[1]贵州师范学院,贵州贵阳550018 [2]山东省高唐县农业局,山东聊城252800 [3]华中农业大学,湖北武汉430070

出　　处：《干旱环境监测》2017年第4期149-153,167,共6页Arid Environmental Monitoring

摘　　要：以悬浮印迹聚合方法合成了毒死蜱分子印迹聚合物,该印迹聚合物对毒死蜱有特异性吸附,对其代谢产物3,5,6-TCP也具有一定的吸附性能,采用该印迹聚合材料作为固相分散萃取剂进行土壤中毒死蜱及3,5,6-TCP的残留测定,结果满足分析要求,方法在0.01~5.0 mg/kg范围内线性关系良好,相关系数为0.998 5~0.999 8;方法的检出限分别为0.571μg/kg(毒死蜱)、0.826μg/kg(3,5,6-TCP);相对标准偏差为1.16%~6.37%;回收率为82.0%~106.8%。该方法可用于土壤中毒死蜱及代谢物3,5,6-TCP的残留测定。The molecularly imprinted polymers（ MIPs） for Chlorpyrifos was synthesized by suspension imprinting polymerization method,which showed a special binding ability for the template and some absorbability to 3,5,6-TCP. The obtained MIPs was used to extract and preconcentrate Chlorpyrifos and its toxic metabolite（ 3,5,6-TCP） as adsorbent of matrix solid phase dispersion from extracting solution of soil,then the samples were detected by high performance liquid chromatography. The results of Chlorpyrifos and 3,5,6-TCP identified in soil samples were satisfactory. The linear correlation was 0. 998 5 - 0. 999 8 between concentrations range of 0. 01 - 5. 0 mg/kg; The detection limits of the method were 0. 571 μg/kg（ Chlorpyrifos） and0. 826 μg/kg（ 3,5,6-TCP） in soil samples,the relative standard deviations were in the range of 1. 16% - 6. 37% in soil samples,the recoveries were in the range of 82. 0% - 106. 8% in soil samples. Those indicated that the proposed method could meet the requirements for simultaneous determination of Chlorpyrifos and its metabolite（ 3,5,6-TCP） in soil.

关 键 词：分子印迹 基质固相分散 高效液相色谱 毒死蜱 3 5 6–TCP 土壤 

分 类 号：X830.2[环境科学与工程—环境工程]