利用微卫星标记技术对红鳍东方鲀(Takifugu rubripes)家系系谱认证的研究  

Parentage Determination of the Takifugu rubripes Families by Microsatellite Markers

作　　者：孙建华[1,2,3] 马爱军 崔文晓 王广宁[2,3] 孙志宾 王新安 孟雪松 刘圣聪 张涛 

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]中国水产科学研究院黄海水产研究所青岛市海水鱼类种子工程与生物技术重点实验室,山东青岛266071 [3]青岛海洋科学与技术国家实验室海洋生物学与生物技术功能实验室,山东青岛266071 [4]大连天正实业有限公司,辽宁大连116000

出　　处：《海洋科学进展》2017年第3期392-403,共12页Advances in Marine Science

基　　金：中央级公益性科研院所基本科研业务费专项--红鳍东方鲀育种群体的遗传差异和生长性状的遗传分析(20603022012005);大连金州新区科技计划项目资助--红鳍东方鲀(Takifugu rubripes)大规模家系选育技术研究(2012-B1-012)

摘　　要：红鳍东方鲀(Takifugu rubripes)是我国北方地区的一种经济型海水鱼类,但目前对其群体遗传多样性和系谱信息研究成果较少。本文研究了目前红鳍东方鲀养殖群体的遗传多样性情况和系谱信息,为其提供遗传改良的理论基础。从2015年所建的12个红鳍东方鲀家系中随机选取5个家系,采用8个微卫星标记对其遗传多样性进行分析和系谱认证。8个位点共检测得到32个等位基因,每个位点等位基因数为36个,平均值为4个。平均观测杂合度(A)范围为0.453 30.953 3,平均期望杂合度(He)范围为0.490 80.790 1,平均多态信息含量(PIC)范围为0.455 50.754 6。不同位点共发现8个特异性等位基因,7#家系发现3个,3#和11#家系各发现2个,12#家系发现1个,10#家系未发现特异性等位基因。根据子代基因型成功推断出5个家系的亲本基因型,据此鉴别各个家系。在fms15位点,可将3#和11#家系与其他家系相区别;TOG01位点可将7#家系与其他家系相区别;f1497位点可将12#家系相区别。因此,fms15,TOG01,f1497这3个标记可以作为鉴别5个家系的特异性标记。结果说明,所选的8个微卫星标记在这5个家系中多态信息含量较高,且在已选用的8个微卫星标记中,最少用3个标记可鉴别5个红鳍东方鲀家系。综合分析表明微卫星标记能有效地为红鳍东方鲀群体的系谱分析提供技术支持,为今后红鳍东方鲀开展分子标记辅助育种提供了可靠的理论依据。Takifugu rubripes is one of the ecnomic sea fish in northern coast of China.In recent years,there are few studies on genetic diversity and genealogical information of Takifugu rubripes.In this study the genetic diversity and genealogical information of Takifugu rubripes were investigated,providing the basis of theory for genetic improvement.Five families were randomly sampled from twelve families which have been established in 2015.Eight pairs of microsatellite primers were used to identify the genealogy and analyze the genetic diversity among five cultured populations of Takifugu rubripes.Thirty-two alleles were detected at eight microsatellite loci.The number of alleles（A）at each locus ranged from three to six with an average of four.The value of average observed heterozygosities was 0.453 30.953 3.The expected heterozygosities（He）was 0.490 8 0.790 1and the mean polymorphic information content（PIC）ranged from 0.455 5to 0.754 6.Eight family unique alleles were found out：three in 7#family,two in 3#family,two in 11#family,one in 12#family.Family unique allele was not found in family 10#.Based on the genotypes of offspring,all parental genotypes of the five families were successfully deduced.3#family and11#family were identified from the other families at locus fms15.7#family was separated from the other families at locus TOG01.12#family was distinguished from the other families at locus f1497.The three microsatellite markers（fms15,TOG01,f1497）could be used to identify five families.Results show that there exists high genetic diversity among the five families.The five families could be identified using at least three microsatellite markers selected from Eight microsatellite markers.Microsatellite marker is an useful tool for genealogical identification of Takifugu rubripes.And it is a reliable basis for molecular marker-assisted breeding in the future.

关 键 词：红鳍东方鲀 微卫星 遗传多样性 系谱认证 

分 类 号：Q953[生物学—动物学]