水飞蓟素抑制草鱼肝细胞脂质蓄积的作用及其机制研究  

INHIBITORY EFFECT OF SILYMARIN ON OLEIC ACID-INDUCED LIPID ACCUMULATION IN GRASS CARP(CTENOPHARYNGODON IDELLUS)HEPATOCYTES IN VITRO

作　　者：萧培珍[1] 吉红[1] 张宝彤[2] 叶元土[3] 孙健[1] 杨洲 李雪贤 田晶晶[1] 

机构地区：[1]西北农林科技大学动物科技学院,杨陵712100 [2]北京市营养源研究所水产动物系统营养研究开放实验室,北京100069 [3]苏州大学基础医学与生命科学学院,苏州215123

出　　处：《水生生物学报》2017年第6期1301-1310,共10页Acta Hydrobiologica Sinica

基　　金：陕西省水产健康生态养殖技术集成与示范(2014-TS-36)资助~~

摘　　要：为探究水飞蓟素(Silymarin)对草鱼肝细胞脂质蓄积的作用及其机理,体外培养草鱼肝细胞,在用400μmol/L油酸对其进行蓄脂诱导的同时,采用不同质量浓度的水飞蓟素处理24h,检测了肝细胞活力、脂质蓄积状况、抗氧化指标和脂质代谢相关基因表达状况。结果显示,水飞蓟素质量浓度在75—125μg/m L对草鱼肝细胞活力无影响(P>0.05);与单独采用油酸进行蓄脂诱导的油酸组比较,水飞蓟素和油酸共同处理的水飞蓟素组中,随着水飞蓟素质量浓度的增加,肝细胞内的脂质含量逐渐降低,且水飞蓟素质量浓度在100—150μg/m L时的肝细胞脂质蓄积水平显著降低(P<0.05);与油酸组相比,100μg/m L水飞蓟素处理组的脂质含量以时间依赖性的模式显著降低(P<0.05),还原型谷胱甘肽含量显著升高(P<0.05),脂肪酸合成酶和硬脂酰辅酶A去饱和酶1的m RNA表达量显著下调(P<0.05)。研究表明,水飞蓟素能够抑制草鱼肝细胞脂质蓄积,其作用可能与其抑制脂质合成基因的表达有关;同时,水飞蓟素可能通过提高肝细胞的抗氧化能力发挥保护肝细胞的作用。The objective of this study was to explore the mechanism and effect of silymarin on hepatocytes lipid accumulation of grass carp（Ctenopharyngodon idellus） induced by oleic acid（OA）. Hepatocytes were treated at 400 μmol/L OA with or without silymarin to observe the cell viability by 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT） and the lipid accumulation by Oil Red O stain. We also tested the effects of silymarin on the antioxidant capacities and the expression of lipid metabolism related gene in hepatocytes in grass carp detected by RT-PCR.The results showed that the cell viability was not affected by the treatment of 400 μmol/L OA with 75—125 μg/m L silymarin in presence for 24 h（P〈0.05）. The intracellular lipid content of hepatocytes decreased significantly after the treatment of 400 μmol/L OA with 100—150 μg/m L silymarin for 24 h by Oil Red O stain（P〈0.05）. Compared with the OA group, SM100 group showed time-dependent trend on the decreasing lipid accumulation（P〈0.05）. Reduced glutathione（GSH） contents were higher in SM100 group than those in the OA group. The expressions of fatty acid synthase（FAS） and stearoyl-Co A desaturases 1（SCD1） m RNA were significantly downregulated in SM100 group compared with those in the OA group（P〈0.05）. These results demonstrated silymarin exerted an inhibitory effect on lipid accumulation by decreasing lipogenesis, and enhanced antioxidative ability in grass carp hepatocytes. Silymarin may shed light on the prevention of fatty liver.

关 键 词：水飞蓟素 脂质蓄积 肝细胞 草鱼 油酸 

分 类 号：S963.7[农业科学—水产养殖]