Fluorescent vital staining of plant sexual cell nuclei with DNA-specific fluorochromes and its application in gametoplast fusion  

作　　者：YANGHONGYUAN XINLIWU 等 

机构地区：[1]DepartmentofBiology,WuhanUniversity,Wuhan430072,China [2]DepartmentofBiology,WuhanUniversity,Wuhan430072

出　　处：《Cell Research》1993年第2期121-130,共10页细胞研究（英文版）

摘　　要：DNA-binding fluorochromes are often used for vital staining of plant cell nuclei. However, it is not always sure whether the cells after staining still remain in living state. We chose several criteria to estimate the validity of real vital staining for sexual cell nuclei. These were: the cytoplasmic streaming in pollen tubes whose nuclei were stained, the simultaneous visualization of fluo-rochromatic reaction and nucleus staining in isolated generative cells, and the capability of isolated, prestained generative or sperm cells to fuse with other protoplasts. The results confirmed that 4',6-diamidino-2-phenylindole (DAPI), Hoechst 33258 and mithramycin could be used as real vital stains, though their efficiency varied from case to case; among them DAPI showed best effect. The fluorescent vital staining technique offered a useful means fori-dentification and selection of heterokaryons in gametoplast manipulation studies.

关 键 词：fluorescent vital staining DNA-specific fluorochrome generative cell sperm cell gametoplast fusion. 

分 类 号：Q943.1[生物学—植物学] Q2－33