力学增强型水凝胶纤维的制备及其生物相容性评价  

Fabrication of Hydrogel Fiber with Enhanced Mechanical Property and Biocompatibility Evaluation

作　　者：汤德锭 师念园[1,3] 常乐 史强 李昱辉[1,3] 徐峰 

机构地区：[1]西安交通大学生命科学与技术学院,西安710049 [2]马鞍山师范高等专科学校,安徽马鞍山243041 [3]西安交通大学生物医学工程与生物力学研究中心,西安710049 [4]安徽中医药高等专科学校,安徽芜湖241002

出　　处：《西安交通大学学报》2017年第12期150-155,共6页Journal of Xi'an Jiaotong University

基　　金：2017年陕西省自然科学基础研究计划资助项目(2017JQ1036);安徽高校自然科学研究重点资助项目(KJ2017A681);博士后创新人才支持计划资助项目(2016)

摘　　要：针对天然水凝胶纤维力学性能及成形性能差的问题,结合微流控和光交联的方法,高通量制备了一种力学增强型海藻酸钠/明胶甲基丙烯酸酯复合水凝胶材料。研究了微流控喷头尺寸、水凝胶单体浓度对水凝胶纤维成形性能、水凝胶力学特性及结构特征的影响。结果表明:制备的水凝胶纤维边缘光滑,直径(390±50μm)较为均一,具有良好的操作性及可控性;随着海藻酸钠和明胶甲基丙烯酸酯单体浓度的增高,水凝胶弹性模量升高,SEM结果显示其片层微结构更为致密。进一步测试了水凝胶纤维的生物相容性,细胞实验结果表明,培养至第5天时,水凝胶中细胞的存活率稳定维持在80%以上,证明所制备的水凝胶具有良好的生物相容性,适宜细胞生长。动物实验结果表明,当凝胶植入动物体内1周后,周围的皮下组织很正常;在第3周时,凝胶周围的炎症反应明显减轻,材料也出现一定程度的降解。研究力学增强型海藻酸钠/明胶甲基丙烯酸酯复合水凝胶,有望于实现三维肌肉微组织模型的高通量构建。Aiming at poor mechanical property and shaping ability of natural hydrogel fiber,combining micro-fluidics with photo-crosslinking,an alginate/GelMA hybrid hydrogel with enhanced mechanical properties in high-throughput manner is fabricated.The impacts of the micro-fluidic nozzle size and the hydrogel monomer concentration on the shaping capability,mechanical property and structural feature of hydrogel are investigated.The fabricated hydrogel fiber is smooth with uniform distribution in diameter(390±50μm)to show the controllability in hydrogel fabrication.SEM results indicate that the hydrogel is endowed with denser lamella microstructure along with increasing concentrations of alginate and GelMA monomer,and theelastic modulus of hydrogel rises accordingly.The biocompatibility of hydrogel fiber is validated by the cell experiments,and the cell viability maintains 80% after 5 days culture.In the animal experiment,ambient subcutaneous tissue remains normal after one week hydrogel implanting,and the surrounding inflammation relieves and hydrogel degrades to some extent in the third week after implanting.This research result of alginate/GelMa hydrogel fiber with enhanced mechanical property is expected to realize high-throughput establishment of three-dimensional muscle microtissue model.

关 键 词：力学增强型水凝胶纤维 高通量制备 海藻酸钠%月胶甲基丙晞酸酯 光交联 微流控 

分 类 号：R318.08[医药卫生—生物医学工程]